Anti-HCN1 Antibody

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
    Cat #: APC-056
    Alternative Name Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0). Intracellular, N-terminus.
        Anti-HCN1 Antibody
    Accession (Uniprot) Number Q9JKB0
    Gene ID 84390
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.
    RRID AB_2039900.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-HCN1 Antibody
        Western blot analysis of rat brain membrane:
        1. Anti-HCN1 Antibody (#APC-056), (1:200).
        2. Anti-HCN1 Antibody, preincubated with the negative control antigen.
        Human HCN1 transfected in HEK-293 cells (Stieber, J. et al. (2005) J. Biol. Chem. 280, 34635.).
      • Anti-HCN1 Antibody
        Expression of HCN1 in mouse cerebellum
        Immunohistochemical staining mouse cerebellum using Anti-HCN1 Antibody (#APC-056). A. HCN1 (green) appears in the cerebellar pinceau. B. Calcium binding calbindinD28-K (red), a marker of Purkinje neurons, is stained in the same section. C. Merge of the images demonstrates the position of the HCN1-positive pinceau structures at the axon initial segment of Purkinje neurons.
      • Anti-HCN1 Antibody
        Expression of HCN1 in rat DRG primary culture
        Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat DRG primary culture. Cells were stained with Anti-HCN1 Antibody (#APC-056), (1:300), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue).
    References
      • Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2

        The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.

        Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2

        The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2HCN3 and HCN4

        HCN1 is extensively expressed in the brain, in specific areas like the neocortex, hippocampus, cerebellum and superior colliculus.2,3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-HCN1 AntibodyExpression of HCN1 in perisoma-inhibiting interneurons (PIIs) of the dentate gyrus.Immunocytochemical staining of perisoma-inhibiting interneurons using Anti-HCN1 Antibody (#APC-056). HCN1 expression (green) is detected in the soma and axon colaterals. Biocytin was used to label neurons.Adapted from Elgueta, C. et al. (2015) with permission of the Society for Neuroscience.
    Last update: 13/01/2020

    Anti-HCN1 Antibody (#APC-056) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize HCN1 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Western blot analysis of rat brain lysate. Tested in HCN1-/- rats.
        Nishitani, A. et al. (2019) Brain Res. 1706, 209.
      • Immunohistochemistry of mouse heart sections. Tested in HCN1-/- mice.
        Herrmann, S. et al. (2011) J. Mol. Cell. Cardiol. 51, 997.
      • Rat brain lysate. Also tested in HCN1-/- rats.
        Nishitani, A. et al. (2019) Brain Res. 1706, 209.
      • Rat synaptosomes.
        Zhang, K. et al. (2016) Sci. Signal. 9, ra123.
      • Human HCN1 transfected in HEK-293 cells.
        Stieber, J. et al. (2005) J. Biol. Chem. 280, 34635.
      • Rat retina sections (1:50).
        Li, Q. et al. (2017) Brain Struct. Funct. 222, 813.
      • Mouse brain sections.
        Matsumoto-Makidono, Y. et al. (2016) Cell Rep. 16, 994.
      • Rat diaphragm sections (1:25).
        Negrini, D. et al. (2016) Am. J. Physiol. 311, H892.
      • Mouse heart sections (1:100).
        Fenske, S. et al. (2013) Circulation 128, 2585.
      • Mouse heart sections. Also tested in HCN1-/- mice.
        Herrmann, S. et al. (2011) J. Mol. Cell. Cardiol. 51, 997.
    Related Products
        • Guinea pig Anti-HCN1 Antibody (#AGP-203) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #APC-056.