Anti-HCN2-ATTO-594 Antibody

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
    Cat #: APC-030-AR
    Alternative Name Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2 (Accession Q9UL51). Intracellular, N-terminus.
        Anti-HCN2-ATTO-594 Antibody
    Accession (Uniprot) Number Q9UL51
    Gene ID 114244
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2341028.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APC-030), and immunohistochemistry (labeled antibody).
    Applications: if, ih
    May also work in: ic*
      • Anti-HCN2-ATTO-594 Antibody
        Expression of HCN2 in rat cerebellum
        Immunohistochemical staining of rat cerebellum using Anti-HCN2-ATTO-594 Antibody (#APC-030-AR). A. HCN2 staining (red) appears in Purkinje neurons. B. Nuclear staining using DAPI as the counterstain (Blue). C. Merged images of A and B.
    References
    • 1. Much, B. et al. (2003) J. Biol. Chem. 278, 43781.
    • 2. Notomi, T. et al. (2004) J. Comp. Neurol. 471, 241.
    • 3. Hofmann, F. et al. (2005) Pharmacol. Rev. 57, 455.
      • Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain having crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2

        The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2

        The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2

        The channels are closely related to each other and share homology of about 60%. However, they are diverging from each other in their cytoplasmic N- and the C-terminus. The channels HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3

        HCN2 is the most abundant neuronal channel and is found almost ubiquitously in the brain.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/11/2019

    Anti-HCN2 Antibody (#APC-030) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize HCN2 from human, rat, and mouse samples.

    Anti-HCN2-ATTO-594 Antibody (#APC-030-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. The conjugated antibody is ideal for immuno-colocalization studies.

    For research purposes only, not for human use
    Citations
      • Immunohistochemical staining of mouse brain sections with #APC-030. Tested in HCN2-/- mice.
        Hammelmann, V. et al. (2011) PLoS ONE 6, e17078.
      • Immunohistochemical staining of mouse heart sections with #APC-030. Tested in HCN2-/- mice.
        Herrmann, S. et al. (2011) J. Mol. Cell. Cardiol. 51, 997.
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