This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
This lyophilized product is shipped at room temperature. Please see its certificate of analysis for further storage instructions.
2. Guinea pig Anti-HCN4 Antibody, preincubated with the control antigen.
- Much, B. et al (2003) J.Biol.Chem. 278, 43781.
- Notomi, T. et al (2004) J. Comp. Neurol. 471, 241.
- Gravante, B. et al (2004) J.Biol.Chem. 279, 43847.
- Moosmang, S. et al. (2001) Eur. J. Biochem. 268, 1646.
- Garcia-Frigola, C. et al. (2003) Gene Expr Patterns 3, 777.
- Monteggia, L. et al. (2000) Molec. Brain Res. 1-2, 129.
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2
The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.
Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2
The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2
The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The HCN1-4 channels mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.
HCN4 is highly expressed in a restricted manner in adult sinoatrial (SA) node, constituting a good molecular marker for the adult cardiac pacemaker and might serve as a unique marker of the developing SA node .4,5
mRNA expression of HCN4 is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus.6
Species reactivity key:
Guinea pig Anti-HCN4 Antibody (#AGP-004), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN4 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-HCN4 Antibody (#APC-052) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
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