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Anti-Homer2 Antibody

Homer protein homolog 2, Cupidin, Vesl-2
Cat #: APZ-027
Alternative Name Homer protein homolog 2, Cupidin, Vesl-2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2 (Accession O88801). Intracellular, C-terminus.
Accession (Uniprot) Number O88801
Gene ID 29547
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756779.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat hippocampus lysate (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat hippocampus lysate (lanes 2 and 4):
    1,2. Anti-Homer2 Antibody (#APZ-027), (1:200).
    3,4. Anti-Homer2 Antibody, preincubated with Homer2 Blocking Peptide (#BLP-PZ027).
  • Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):
    Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):
    1,2. Anti-Homer2 Antibody (#APZ-027), (1:200).
    3,4. Anti-Homer2 Antibody, preincubated with Homer2 Blocking Peptide (#BLP-PZ027).
Immunohistochemistry
  • Expression of Homer2 in mouse cerebellum and rat hippocampus
    Expression of Homer2 in mouse cerebellum and rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-Homer2 Antibody (#APZ-027), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. Homer2 staining (green) in mouse cerebellum is observed in Purkinje cells (arrows) and their dendrites. B. Homer2 staining (green) in rat hippocampal dentate gyrus region is detected granule layer (GL) and interneurons in the hilus (arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Hayashi, M.K. et al. (2006) J. Neurosci. 26, 8492.
  2. Shiraishi, Y. et al. (2003) J. Neurochem. 87, 364.
  3. Guo, W. et al. (2015) Cell Rep. 13, 2297.
  4. Smothers, C.T. et al. (2016) Synapse 70, 33.
Scientific background

Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.  

The Homer family includes three members, Homer1b/c, Homer2a/b, and Homer3. Homer2 protein, also called Vesl-2, is a scaffolding protein that is important for the proper organization, localization and signaling of excitatory postsynaptic receptors. Homer2 protein associates with filamentous actin (F-actin) and directly binds to drebrin, an actin-binding and remodeling protein in dendritic spines. The protein also binds to activated Cdc42, a member of the Rho small GTPase family2. The protein interacts with mGluR1mGluR5, PI3 Kinase enhancer (PIKE), inositol 1,4,5-trisphosphate receptors (IP3), SHANK and other proteins3,4.

Deletion of the Homer2 gene in mice demonstrates several abnormalities in glutamatergic signaling and in the nucleus accumbens such as reduced basal extracellular glutamate content and reduced function and total protein expression of both mGluR1 and the cystine-glutamate exchanger4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Homer2 Antibody (#APZ-027) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer2 from human, mouse, and rat samples.

For research purposes only, not for human use
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