Anti-Human ASCT2/SLC1A5 (extracellular) Antibody

Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
  • New
Cat #: ANT-083
Alternative Name Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    Immunogen
    • Peptide (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2 (Accession Q15758). 2nd extracellular loop.
    • Anti-Human ASCT2/SLC1A5 (extracellular) Antibody
    Accession (Uniprot) Number Q15758
    Gene ID 6510
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Specificity The antibody will not recognize rat or mouse ASCT2.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human ASCT2/SLC1A5 (extracellular) Antibody
      Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 5), human Colo-205 colorectal adenocarcinoma (lanes 2 and 6), human PANC-1 pancreas ductal adenocarcinoma (lanes 3 and 7) and human Malme-3M melanoma cell line lysate (lanes 4 and 8):
      1-4. Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (#ANT-083), (1:200)
      5-8. Anti-Human ASCT2/SLC1A5 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human ASCT2/SLC1A5 (extracellular) Antibody
      Cell surface detection of ASCT2 in live intact human HL-60 promyelocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (#ANT-083), (2.5 µg) + goat-anti-rabbit-FITC.
    References
    1. Kasai, N. et al. (2017) Am. J. Transl. Res. 9, 3399.
    2. Marshall, A.D. et al. (2017) Oncogenesis 6, e367.
    3. Dong, J. et al. (2017) Oncogenesis 6, e356.
    Scientific background

    ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.

    ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways.  It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 28/06/2020

    Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (#ANT-083) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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