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Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody

C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182

Cat #: ACR-012-AG
Alternative Name C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
  • Peptide (C)EDFNMESDSFEDFWKGED, corresponding to amino acid residues 2-19 of human CXCR2 (Accession P25025). Extracellular, N-terminus.
Accession (Uniprot) Number P25025
Gene ID 3579
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only.
RRID AB_10660846.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will only recognize CXCR2 from human samples.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-012), and direct flow cytometry (labeled antibody).
Applications: fc, lci
May also work in: ic*, ih*
Direct flow cytometry
  • Cell surface detection of CXCR2 in live intact Jurkat cells:
    Cell surface detection of CXCR2 in live intact Jurkat cells:
    ___ Unstained cells.
    ___ Cells + Anti-CXCR2 (extracellular)-ATTO Fluor-488 Antibody, (#ACR-012-AG), (5-10 µg/5x105 cells).
  1. Jin, T. et al. (2008) Cytokine 44, 1.
  2. Murphy, P.M. (2002) Pharmacol. Rev. 54, 227.
  3. Viola, A. and Luster, A.D. (2007) Annu. Rev. Pharmacol. Toxicol. 48, 171.
  4. Cardona, A.E. et al. (2008) J. Leukoc. Biol. 84, 587.
  5. Lodowski, D.T. and Palczewski, K. (2009) Curr. Opin. HIV AIDS 4, 88.
  6. Tran, P.B. and Miller, R.J. (2003) Nat. Rev. Neurosci. 4, 444.
  7. Moser, B. and Loetscher, P. (2001) Nat. Immunol. 2, 123.
  8. Pals, S.T. et al. (2007) Blood 110, 3102.
  9. Waugh, D.J.J. and Wilson, C. (2008) Clin. Cancer Res. 14, 6735.
  10. Page, E.L. et al. (2002) J. Biol. Chem. 277, 48403.
  11. Horuk, R. et al. (1997) J. Immunol. 158, 2882.
  12. Dunstan, C.A. et al. (1996) J. Biol. Chem. 271, 32770.
Scientific background

Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2.

All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition3.

Chemokines were first identified by their ability to mediate leukocyte chemoattraction. Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity4-6. Indeed, chemokines could also be further classified as being inflammatory, as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells7,8.

In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, namely breast cancer1. The chemokine signaling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection.

The CXCR2 receptor could mediate chemotaxis and degranulation of neutrophils along with CXCR1 via interleukin-8 (IL-8), a proinflammatory chemokine with the CXC motif9. In addition, in a model of arterial injury, CXCR2 can improve endothelial recovery, and also seems to be important in recruiting endothelial progenitor cells to injured vessels10. CXCR2 is activated by a plethora of ligands: GROa, b, g, neutrophil-activating peptide, granulocyte chemotactic protein-2 and keratinocyte derived chemokine (CXCL1). CXCR2 is expressed in different brain regions11, in the lung and spleen12 and other regions.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2022

Anti-Human-CXCR2 (extracellular) Antibody (#ACR-012) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect flow cytometry applications, and has been designed to recognize CXCR2 from human samples.

Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody (#ACR-012-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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