Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody

N-formyl peptide receptor 1, fMLP receptor

Cat #: AFR-001-AG
Alternative Name N-formyl peptide receptor 1, fMLP receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192  of human FPR1 (Accession P21462). 2nd extracellular loop.
Accession (Uniprot) Number P21462
Gene ID 2357
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only. Not recommended for use with rat and mouse samples.
RRID AB_11218953.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AFR-001) and flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, ih*, lci*
Direct flow cytometry
  • Cell surface detection of FPR1 in live intact human THP-1 acute monocytic leukemia cells:
    Cell surface detection of FPR1 in live intact human THP-1 acute monocytic leukemia cells:
    ___ Unstained cells.
    ___ Cells + Anti-Human FPR1 (extracellular)-ATTO-488 Antibody (#AFR-001-AG), (1:20).
References
  1. Ye, R.D. et al. (2009) Pharmacol. Rev. 61, 119.
  2. Le, Y. et al. (2002) Trends Immunol. 23, 541.
  3. Murphy, P.M. et al. (1992) J. Biol. Chem. 267, 7637.
  4. Becker, E.L. et al. (1998) Cell Tissue Res. 292, 129.
Scientific background

Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.

FPRL1, or FPR2/ALX as it is commonly called, is a seven transmembrane protein like all GPCRs. This receptor was originally cloned by screening a HL60 neutrophil cDNA library with a FPR1 cDNA probe3. FPR2/ALX shares 69% identity with FPR1 and despite its high homology, it displays relatively low affinity for fmlf, the most potent N-formyl peptide released by bacteria3.

FPR1 was originally found in neutrophils and later found to be distributed in myeloid and non-myeloid cells as is the case for FPR2/ALX and FPR3 (FPR3 though is not expressed in neutrophils). FPR1 is also expressed in multiple organs and tissues including epithelial cells in organs with secretory functions, endocrine cells, liver hepathocytes, smooth muscle cells and endothelial cells, brain spinal cord and both motor and sensory neurons4. FPR2/ALX has a similar tissue distribution to that of FPR1.

While N-formyl peptides were the first peptides found to activate these receptors, the ligand diversity for FPR has proven to be quite broad and demonstrates to be both pro- and anti-inflammatory. They include peptidic ligands originating from bacterial and viral sources (including HIV), endogenous ligands such as chemokines and annexins, short peptides associated with inflammation and infection. Indeed, peptides from Herpes, Ebola and coronavirus 229E are ligands of FPR11.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Human FPR1 (extracellular) Antibody (#AFR-001) is a highly specific antibody directed against an epitope of the human N-formyl peptide receptor 1. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize FPR1 from human samples only.

Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody (#AFR-001-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information