Anti-Human GPR43/FFAR2 (extracellular) Antibody

G-protein coupled receptor 43, Free fatty acid receptor 2
    Cat #: AFR-015
    Alternative Name G-protein coupled receptor 43, Free fatty acid receptor 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    • Peptide (C)TEQVRSGNEIT(S)YEN, corresponding to amino acid residues 153-167 of human FFAR2 (Accession O15552). 2nd extracellular loop.
    • Anti-Human GPR43/FFAR2 (extracellular) Antibody
    Accession (Uniprot) Number O15552
    Gene ID 2867
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for mouse or rat samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human GPR43/FFAR2 (extracellular) Antibody
      Western blot analysis of human HT-29 colon adenocarcinoma cell line (lanes 1 and 5), human HL-60 promyelocytic leukemia cell line (lanes 2 and 6), human THP-1 monocytic leukemia cell line (lane 3 and 7) and human LoVo colorectal adenocarcinoma cell line (lanes 4 and 8) lysates:
      1-4. Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015), (1:200).
      5-8. Anti-Human GPR43/FFAR2 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human GPR43/FFAR2 (extracellular) Antibody
      Cell surface detection of FFAR2 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Kimura, I. et al. (2014) Front. Endocrinol. 5, 85.
    2. Hong, Y.H. et al. (2005) Endocrinology 146, 5092.
    3. Sivaprakasam, S. et al. (2016) Oncogenesis 5, e238.
    4. Galvao, I. et al. (2018) Front. Immunol. 9, 142.
    5. Montalvany-Antonucci, C.C.  et al. (2019) Bone 125, 112.
    6. Sina, C. et al. (2009) J. Immunol. 183, 7514.
    7. Maslowski, K.M. et al. (2009) Nature 461, 1282.
    Scientific background

    Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.

    FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.

    GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.

    These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.

    Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6,7. The various activities mediated by GPR43 makes it an appealing therapeutic target.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015) is a highly specific antibody directed against an epitope of the human Free fatty acid receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FFAR2 from human samples only. It will not recognize the protein from rat or mouse samples.

    For research purposes only, not for human use



    Scientific Background


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