Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody

H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35
    Cat #: AHR-004
    Alternative Name H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    Immunogen
    • Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4 (Accession Q9H3N8). 1st extracellular loop.
    • Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody
    Accession (Uniprot) Number Q9H3N8
    Gene ID 59340
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Won't recognize rat or mouse H4 Histamine Receptor.
    RRID AB_2039921.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.7 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody
      Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates:
      1,2. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).
      3,4. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody
      Cell surface detection of Histamine H4 receptor in live intact Jurkat (acute T-cell leukemia) cell lines:
      ___ Unstained cells + goat-anti-rabbit-PE.
      ___ Cells+ Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:20) + goat-anti-rabbit-PE.
    • The negative control antigen is not suitable for this application.
    References
    1. Hough, L.B. (2001) Mol. Pharmacol. 59, 415.
    2. Thurmund, R.L. et al. (2008) Nat. Rev. Drug. Discov. 7, 41.
    3. Zampeli, E. and Tiligada, E. (2009) Br. J. Pharmacol. 157, 24.
    Scientific background

    Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1

    The biological effects of histamine are mediated through four receptors (H1- H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    Histamine H4 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.1,2

    Histamine H4 receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2,3

    These studies indicate that histamine H4 receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2,3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 1st extracellular loop of the human histamine H4 receptor. AntiHuman Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004) can be used in western blot and indirect flow cytometry applications, and recognizes H4R from human samples.

    For research purposes only, not for human use
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