Anti-Human ABCB1/MDR1 Antibody

Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170
Cat #: AMT-021
Alternative Name Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    Immunogen
    • Peptide (C)RGSQAQDRKLSTKE, corresponding to amino acid residues 673-686 of human multidrug resistance protein 1 (Accession P08183). 3rd intracellular loop.
    • Anti-Human ABCB1/MDR1 Antibody
    Accession (Uniprot) Number P08183
    Gene ID 5243
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Not recommended for rat or mouse samples.
    RRID AB_2756673.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for rat or mouse samples.
    The antibody is specific for MDR1. It won’t cross-react with the highly homologous MDR3 protein.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-Human ABCB1/MDR1 Antibody
      Western blot analysis of human LNCaP prostate carcinoma (lanes 1 and 4), human HeLa cervix carcinoma (lanes 2 and 5) and human HepG2 liver carcinoma (lanes 3 and 6) cell line lysates:
      1-3. Anti-Human ABCB1/MDR1 Antibody (#AMT-021), (1:200).
      4-6. Anti-Human ABCB1/MDR1 Antibody, preincubated with the negative control antigen.
    References
    1. Cole, S.P. (2014) J. Biol. Chem. 289, 30880.
    2. Greenberg, R.M. (2014) Int. J. Parasitol. Drugs. Drug. Resist. 4, 301.
    Scientific background

    Multidrug resistance protein 1 (MRP1, also known as P glycoprotein 1) is a member of a superfamily of proteins known as the ATP-binding cassette (ABC) transporters. This superfamiy is classified into seven subfamilies (A through G). Subfamily ABCC is composed of 12 proteins and includes MRP1 and is encoded by ABCC1.

    MRP1 structure comprises five domains with two nucleotide binding domains (NBDs) that bind ATP, and 12 transmembrane domains.

    MRP1 mediates the ATP-dependent efflux of a variety of solutes across membranes against a concentration gradient. These solutes include antineoplastic agents such as vincristine and doxorubicin, physiological organic anions such as the antioxidant GSH and the pro-inflammatory cysteinyl leukotriene C4, hydrophilic conjugated xenobiotic, various antibiotics, opiates, antiviral agents, citalopram, and statins. MRP1 is the only MRP-related protein with an acknowledged role in tumor multidrug resistance in clinical oncology. It also plays an important role in the efficacy and toxicity of drugs used for the treatment of nonmalignant diseasese1,2.

    Knock-out mouse model of Abcc1−/− has shown that MRP1 can act as an important factor of drug disposition because of its presence in cells at the interface between many tissues. The protein also demonstrates chemoprotective properties1.

    Evidence suggests that ABC transporters also have an important role in normal cellular biology including regulation of cell differentiation, proliferation, and survival, including apoptosis, tumor promotion and modulation of immune function2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human multidrug resistance protein 1. Anti-Human ABCB1/MDR1 Antibody (#AMT-021) can be used in western blot analysis. It has been designed to recognize MDR1 from human samples only. The antibody is specific for MDR1 and will not cross-react with the highly homologous MDR3 protein.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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