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Anti-Human TRPV1 (extracellular) Antibody

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1

Cat #: ACC-334
Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)RPVDGLPPFKMEKTGD, corresponding to amino acid residues 456 - 471 of human TRPV1 (Accession Q8NER1). Extracellular, 1st loop.
Accession (Uniprot) Number Q8NER1
Gene ID 7442
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Not recommended for rat and mouse samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human LNCaP prostate adenocarcinoma cell line lysate (lanes 2 and 5) and human HMC3 microglia cell line lysate (lanes 3 and 6):
    Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human LNCaP prostate adenocarcinoma cell line lysate (lanes 2 and 5) and human HMC3 microglia cell line lysate (lanes 3 and 6):
    1-3. Anti-Human TRPV1 (extracellular) Antibody (#ACC-334), (1:500).
    4-6. Anti-Human TRPV1 (extracellular) Antibody, preincubated with Human TRPV1 (extracellular) Blocking Peptide (BLP-CC334).
Indirect flow cytometry
  • Cell surface detection of TRPV1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of TRPV1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Human TRPV1 (extracellular) Antibody (#ACC-334), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
  2. Cesare, P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 7658.
  3. Kim, J. et al. (2003) Nature 424, 81.
  4. Ahern, G. P. et al. (2003) J. Biol. Chem. 278, 30429.
  5. Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790.
  6. Tominaga, M. et al. (1998) Neuron 21, 531.
  7. Vlachova, V. et al. (2003) J. Neurosci. 23, 1340.
  8. Agopyan, N. et al. (2004) Am. J. Physiol. 286, L563.
  9. Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170.
Scientific background

TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.

The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3

TRPV1 has many activators among them heat, protons, vanilloids like capsaicinresiniferatoxin (RTX), and lipids.4 This channel is associated with tissue injury and inflammation.5,6

Other members of this family, TRPV2TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.

There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 19/04/2021

Anti-Human TRPV1 (extracellular) Antibody (#ACC-334) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and flow cytometry applications.
This antibody is not recommended for rat and mouse samples.

For research purposes only, not for human use
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