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Anti-IBA1/AIF1 Antibody

Ionized calcium-binding adapter molecule 1, MRF-1, Microglia response factor
Cat #: ACS-010
Alternative Name Ionized calcium-binding adapter molecule 1, MRF-1, Microglia response factor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)DEDLQSKLEAFKTK, corresponding to amino acid residues 40-53 of rat IBA1/AIF1 (Accession P55009). Intracellular.
Accession (Uniprot) Number P55009
Gene ID 29427
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 13/14 amino acid residues identical; human – 11/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat spleen (lanes 1 and 3), rat testis (lanes 2 and 4) and mouse spleen (lanes 5 and 6) lysates:
    Western blot analysis of rat spleen (lanes 1 and 3), rat testis (lanes 2 and 4) and mouse spleen (lanes 5 and 6) lysates:
    1, 2. Anti-IBA1/AIF1 Antibody (1:200), (#ACS-010).
    3, 4. Anti-IBA1/AIF1 Antibody, preincubated with IBA1/AIF1 Blocking Peptide (#BLP-CS010).
Immunohistochemistry
  • Expression of IBA1/AIF1 in rat brain
    Expression of IBA1/AIF1 in rat brain
    Immunohistochemical staining of rat free floating frozen brain sections using Anti-IBA1/AIF1 Antibody (#ACS-010). A. In hippocampal CA1 region, IBA1 immunoreactivity (red) is observed in microglia. B. In hippocampal dentate gyrus region IBA1 staining (red) also appears in microglia. Nuclei are stained using DAPI as the counterstain (blue).
References
  1. Zhao, Y.Y. et al. (2013) Cell. Immunol. 284, 75.
  2. Liu, G. et al. (2007) Autoimmunity 40, 95.
  3. Ito, D. et al. (1998) Brain Res. Mol. Brain Res. 57, 1.
  4. Chen, Z.W. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 13879.
Scientific background

Allograft Inflammatory Factor-1 (AIF1) is a 17 kDa. cytoplasmic, calcium and actin binding scaffold protein, mainly expressed in immunocytes. AIF-1 is induced by cytokines such as interferon-γ (IFN-γ).

It is encoded within the HLA class III genomic region by IBA1 gene, and its structure contains seven alpha helixes and three beta sheets. There are three other proteins identical to AIF1: Ionized Ca2+-Binding Adaptor 1, Microglia Response Factor-1, and Daintain1.

The gene is highly expressed in testis, spleen, circulating macrophages and in the brain but weakly expressed in lung and kidney. Among brain cells, the Iba1 gene is strongly and specifically expressed in microglia and its expression is up-regulated following nerve injury, central nervous system ischemia, and several other brain diseases2.

AIF1 influences the immune system at several key points and thus modulates inflammatory diseases. It increases the expression of inflammatory mediators such as cytokines, chemokines, inducible nitric oxide synthase (iNOS) and promotes inflammatory cell proliferation and migration and hence it is highly associated with autoimmune diseases3. In addition, its abundance is positively correlated with metabolic indicators, such as body mass index, triglycerides, and fasting plasma glucose levels. There is also evidence that AIF1 could be a marker for diabetic nephropathy when detected in serum. It is found in activated macrophages in the pancreatic islets, and has been shown to decrease insulin secretion, while simultaneously impairing glucose elimination4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:
Multiplex staining of IBA1/AIF1 and P2X7 in rat brain.
Immunohistochemical staining of rat corpus callosum (CC) free floating frozen sections using Anti-IBA1/AIF1 Antibody (#ACS-010), (1:1000) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO) (1:60). A. IBA1/AIF1 immunoreactivity (green) appears in microglia (arrows). B. P2X7 immunostaining (red) appears in microglia (up-pointing arrows) and in other cell types in the corpus callosum (down-pointing arrows). C. Merged image of panels A and B demonstrates partial colocalization of both proteins. Nuclei are demonstrated using DAPI as the counterstain (blue).
Last update: 23/08/2020

Anti-IBA1/AIF1 Antibody (#ACS-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IBA1 from human, mouse, and rat samples.

For research purposes only, not for human use
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