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Home › Products › Ion Channels › K+ Channels › Two-Pore Domain K+ Channels › Antibodies

Certificate of Analysis

Anti-KCNK2 (TREK-1) Antibody

Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1

Cat #: APC-047
Alternative Name Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
  • KO Validated
    The specificity of this antibody has been validated in a knockout (KO) or knockdown (KD) system.
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069). Intracellular, N-terminus.
    Accession (Uniprot) Number O95069
    Gene ID 3776
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; mouse - 17/18 amino acid residues identical.
    RRID AB_2040136.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Rat brain membranes (1:200).
    • Western blot analysis of rat brain membranes:
      Western blot analysis of rat brain membranes:
      1. Anti-KCNK2 (TREK-1) Antibody (#APC-047), (1:200).
      2. Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).
    Immunohistochemistry
    • Rat hippocampus sections (Banerjee, A. et al. (2016) J. Neurochem. 138, 265.).
    Immunocytochemistry
    • Mouse primary cortical astrocytes (1:100) (Hwang, E.M. et al. (2014) Nat. Commun. 5, 3227.).
    Scientific background

    KCNK2 (also named TWIK-related K+ channel, TREK-1 or K2P2.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.

    The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. 

    K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK2 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, KCNK2 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.

    KCNK2 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while KCNK2 expression in rodents is more widespread.

    KCNK2 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that KCNK2 may be a potential target for anti-depressants.

    KCNK2 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gαi, dissociation of Gβγ, followed by the opening of the glutamate permeable KCNK2 (TREK-1) K+ channel through its interaction with Gβγ. For this purpose, KCNK2 is localized at the cell surface of the cell body and processes of astrocytes.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized Powder
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    Image & Title:

    Anti-KCNK2 (TREK-1) Antibody
    Knockout validation of Anti-KCNK2 (TREK-1) Antibody in mouse hippocampus and expression of TREK-1 in mouse astrocytes. Immunohistochemical staining of mouse brain sections using Anti-KCNK2 (TREK-1) Antibody (#APC-047). A. TREK-1 staining (green) co-localizes with µ-opioid receptor (red) in astrocytes in CA1 region. B. TREK-1 expression (green) in mouse hippocampus (left panel). Lack of TREK-1 staining in TREK-1-/- mice confirms antibody specificity (right panel).Adapted from Woo, D.H. et al. (2018) Front. Cell. Neurosci. 12, 319. with permission of Frontiers.

    Last update: 08/01/2023

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    For research purposes only, not for human use

    Specifications

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    Scientific Background

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      Control Product

      • KCNK2/TREK-1 Blocking Peptide (#BLP-PC047) is the original antigen used for immunization during Anti-KCNK2 (TREK-1) Antibody (#APC-047) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK2/TREK-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

        KCNK2/TREK-1 Blocking Peptide (#BLP-PC047)

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