Anti-GRIK1 (GluK1) (extracellular) Antibody

Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5
    Cat #: AGC-008
    Alternative Name Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: r
    May also work in: h*, m*
      • Peptide (C)KASGEVSKHLYKVWKK, corresponding to amino acid residues 402-417 of rat kainate receptor GluK1 (Accession P22756). Extracellular, N-terminus.
        Anti-GRIK1 (GluK1) (extracellular) Antibody
    Accession (Uniprot) Number P22756
    Gene ID 29559
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, dog, monkey - 15/16 amino acid residues identical.
    RRID AB_2039896.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-GRIK1 (GluK1) (extracellular) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:200).
        2. Anti-GRIK1 (GluK1) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Anti-GRIK1 (GluK1) (extracellular) Antibody
        Expression of GluK1 in rat cerebellum
        Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:400), followed by donkey anti rabbit-Cy3 antibody. GluK1 staining (red) appears in the granule layer and in the baskets under Purkinje cells (arrows). Nuclei were labeled with DAPI and appear in blue.
      • Anti-GRIK1 (GluK1) (extracellular) Antibody
        Expression of kainate receptor GluK1 in rat DRG neurons
        Cell surface detection of kainate receptor GluK1 in living rat dorsal root ganglion (DRG). A. Extracellular staining of cells using Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the same field.
    References
      • L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).

        The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.

        The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2

        Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing; as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2

        GluR5 is highly expressed in dorsal root ganglion (DRG) neurons of the peripheral nervous system (PNS) as well as in several structures of the CNS including the amygdala, the hipoccampus, and Purkinje cells of the cerebellum.

        GluR5 has been implicated in the modulation of synaptic transmission and synaptic plasticity in both the CNS and PNS, but the exact physiological function of GluR5 is not well understood. Nevertheless, an involvement in persistent pain and some types of learning has been proposed.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Alomone Labs is pleased to offer Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), a highly specific antibody directed against the extracellular N-terminus domain of the rat kainate receptor GluK1. The antibody can be used in western blot and immunofluorescence applications.

    For research purposes only, not for human use