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Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
Cat #: ALM-051-B
Alternative Name IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
Lyophilized Powder yes
Type: Monoclonal
Host: Mouse
Reactivity: h, m, r
Immunogen
  • Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).
Accession (Uniprot) Number O15554
Gene ID 3783
RRID AB_2340987.
Purity Affinity purified from cultured hybridoma medium.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype IgM.
Label Biotin.
Clone 6C1.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: fc, ic, if, lci
May also work in: ih*
Direct flow cytometry
  • Cell surface detection of KCNN4 in live intact human THP-1 acute monocytic leukemia cell line:
    Cell surface detection of KCNN4 in live intact human THP-1 acute monocytic leukemia cell line:
    ___ Cells + Streptavidin-AlexaFluor-647.
    ___ Cells + Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody (#ALM-051-B), (1:10) + Streptavidin-AlexaFluor-647.
Live cell imaging / Immunocytochemistry
  • Expression of KCNN4 in live LN-CaP cells
    Expression of KCNN4 in live LN-CaP cells
    Cell surface detection of KCNN4 in live intact human LNCaP prostate carcinoma cells. A. Cells were stained with Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody (#ALM-051-B), (1:15), followed by Streptavidin-AlexaFluor-594 (red). B. Cell nuclei were labeled with the cell permeable dye Hoechst 33342 (blue). C. Merge of the two images.
References
  1. Ghanshani, S. et al. (2000) J. Biol. Chem. 275, 37137.
  2. Hoffman, J.L. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7366.
Scientific background

KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. 

KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel.  

The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 16/08/2020

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples.

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody (#ALM-051-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody has been tested in direct flow cytometry and immunocytochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

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For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunocytochemistry citations
  1. Human CD8+ T-cells.
    Chimote, A.A. et al. (2020) Front. Pharmacol. 11, 143.

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