Guinea pig Anti-Kir2.1 (KCNJ2) Antibody

Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
    Cat #: AGP-044
    Alternative Name Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: h, m, r
      • Peptide (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1 (Accession P48049). Intracellular, C-terminus.
        Guinea pig Anti-Kir2.1 (KCNJ2) Antibody
    Accession (Uniprot) Number P48049
    Gene ID 3759
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rabbit, bovine, pig, guinea pig - identical; rat, mouse - 17/19 amino acid residues identical; chicken - 15/19 amino acid residues identical.
    RRID AB_2340970.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Guinea pig Anti-Kir2.1 (KCNJ2) Antibody
        Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
        1-2. Guinea pig Anti-Kir2.1 (KCNJ2) Antibody (#AGP-044), (1:500).
        3-4. Guinea pig Anti-Kir2.1 (KCNJ2) Antibody, preincubated with the negative control antigen.
        Guinea pig Anti-Kir2.1 (KCNJ2) Antibody
        Western blot analysis of human U-87 MG glioblastoma cell lysate:
        1. Guinea pig Anti-Kir2.1 (KCNJ2) Antibody (#AGP-044), (1:500).
        2. Guinea pig Anti-Kir2.1 (KCNJ2) Antibody, preincubated with the negative control antigen.
    References
      • Kir2.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

        The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.

        Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.

        Kir2.1 is a member of the Kir2.x subfamily that includes four members (Kir2.1- Kir2.4) that are characterized by strong inward rectification and high constitutive activity.

        Kir2.1 is expressed in a variety of tissues including the heart, brain, vascular smooth muscle cells and skeletal muscles.

        In the heart, Kir2.1 is a molecular component of the IK1 current that is responsible for setting the resting membrane potential, preventing membrane hyperpolarization due to Na+ pump activity, influencing propagation velocity, altering the electrical space constant, and promoting late phase repolarization.2 In fact, mutations in Kir2.1 channels have been linked to a form of long QT syndrome (LQT7) known as Andersen's syndrome that is characterized by cardiac arrhythmias, periodic paralysis, and dysmorphic features.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Kir2.1 channel. Guinea pig Anti-Kir2.1 (KCNJ2) Antibody (#AGP-044) raised in guinea pig can be used in western blot analysis. It was designed to recognize Kir2.1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir2.1 (KCNJ2) Antibody (#APC-026) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use