Overview
Cat #:
APC-042
Alternative Name Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.
Accession (Uniprot) Number P52188
Gene ID 117052
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.
RRID AB_2040109.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.39 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200).
3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042).- Human retinal pigment epithelium (1:300) (Yang, D. et al. (2008) Exp. Eye Res. 87, 176.).
Immunohistochemistry
Expression of Kir2.2 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Kir2.2 immunoreactivity (green) appeared in Bergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows). B. Pre-incubation of the antibody with Kir2.2/KCNJ12 Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer.
Immunocytochemistry
- Neonatal rat cardiomyocytes (NRCMs) (1:200), (He, Y. et al. (2008) FEBS Lett. 582, 2338.).
References
- Nichols, C.G. and Lopatin, A.N. (1997) Annu. Rev. Physiol. 59, 171.
- Preisig-Muller, R. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 7774.
Scientific background
Kir2.2 is a member of the inward rectifying K+ channel family. The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4. The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+.
The Kir2.2 subunit is expressed in the CNS, kidney and heart where it has been found to form heteromeric channels with Kir2.1.
Lyophilized Powder
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat IRK-2 channel. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042) can be used in western blot, and immunocytochemistry applications. It has been designed to recognize IRK-2 from human, rat and mouse samples.
For research purposes only, not for human use
Last Update: 03/01/2024
Applications
Citations
Citations
Powered by BiozWestern blot citations
- Human THP-1 cells.
Kim, K.S. et al. (2015) J. Immunol. 195, 3345. - Canine heart lysate.
Bonilla, I.M. et al. (2012) J. Appl. Physiol. 113, 1772. - Human retinal pigment epithelial (1:300).
Yang, D. et al. (2008) Exp. Eye Res. 87, 176.
