- Peptide (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.
- Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200).
3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042).
- Human retinal pigment epithelium (1:300) (Yang, D. et al. (2008) Exp. Eye Res. 87, 176.).
- Expression of Kir2.2 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Kir2.2 immunoreactivity (green) appeared in Bergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows). B. Pre-incubation of the antibody with Kir2.2/KCNJ12 Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer.
- Neonatal rat cardiomyocytes (NRCMs) (1:200), (He, Y. et al. (2008) FEBS Lett. 582, 2338.).
Kir2.2 is a member of the inward rectifying K+ channel family. The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4. The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+.
The Kir2.2 subunit is expressed in the CNS, kidney and heart where it has been found to form heteromeric channels with Kir2.1.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat IRK-2 channel. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042) can be used in western blot, and immunocytochemistry applications. It has been designed to recognize IRK-2 from human, rat and mouse samples.