Alternative Name Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12
Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Antigen Incl. Antigen Incl.
This antibody is shipped with its antigen FREE of charge!
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
ImmunogenPeptide (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat K
ir2.2 (Accession
P52188). Intracellular, C-terminal part.
Accession (Uniprot) Number P52188
Gene ID 117052
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.
RRID AB_2040109.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.39 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Negative control antigen reconstitution 100 µl double distilled water (DDW).
Negative control antigen storage after reconstitution -20°C.
Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ic, if, wb
May also work in: ifc*, ih*, ip*
Western blotRat brain and kidney membranes (1:200).
Human retinal pigment epithelium (1:300) (
Yang, D. et al. (2008)
Exp. Eye Res. 87, 176.).
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:
1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200).
3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with the control peptide antigen.
ImmunocytochemistryNeonatal rat cardiomyocytes (NRCMs) (1:200), (
He, Y. et al. (2008)
FEBS Lett. 582, 2338.).
Scientific background Kir2.2 is a member of the inward rectifying K+ channel family. The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4. The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+.
The Kir2.2 subunit is expressed in the CNS, kidney and heart where it has been found to form heteromeric channels with Kir2.1.
Lyophilized PowderNegative Control Antigen Included
Last update: 24/01/2020
For research purposes only, not for human use
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200).