Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
    Cat #: APC-035-AG
    Alternative Name ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KLEESLREQAEKEGSALSVR, corresponding to residues 356-375 of rat Kir4.1 (Accession P49655). Intracellular, C-terminus.
    • Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody
    Accession (Uniprot) Number P49655
    Gene ID 29718
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - identical.
    RRID AB_2040118.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APC-035), and immunohistochemistry (labeled antibody).
    Applications: if, ih
    May also work in: ic*
    Immunohistochemistry
    • Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody
      Expression of Kir4.1 in rat cerebellum
      Immunohistochemical staining of frozen sections of rat cerebellum using Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody (#APC-035-AG) (green), (1:50). Staining is specific for Bergmann glial cells prolongations (white arrows) in the molecular layer (ML) and astrocytes (yellow arrows) in the granular layer (GL). Purkinje cell bodies are stained with fluorescent Nissl stain (red). Hoechst 33342 (blue) is used as counterstain.
    • Mouse brain sections (1:400) (Steiner, E. et al. (2012) Glia 60, 1646.).
    References
    1. Takumi, T. et al. (1995) J. Biol. Chem. 270, 16339.
    2. Higashi, K. et al. (2001) Am. J. Physiol. 281, C922.
    3. Butt, A.M. and Kalsi, A. (2006) J. Cell Mol. Med. 10, 33.
    Scientific background

    Kir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

    The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.

    Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.

    Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.

    The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. 

    Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney.1,2

    It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the Kreleased during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Kir4.1 potassium channel from rat, mouse, and human samples.

    Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody (#APC-035-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Immunohistochemical staining of mouse brain sections with #APC-035. Tested in Kir4.1-/- mice.
      Brasko, C. et al. (2017) Brain Struct. Funct. 222, 41.
    2. Immunohistochemical staining of mouse oligodendrocyte sections with #APC-035. Tested on Kir4.1-/- cells.
      Battefeld, A. et al. (2016) Nat. Commun. 7, 11298.
    3. Immunohistochemical staining of mouse cerebellum sections with #APC-035. Tested in Kir4.1 conditional knockout cerebellum.
      Djukic, B. et al. (2007) J. Neurosci. 27, 11354.
    Immunohistochemistry citations
    1. Mouse brain sections (1:200).
      Steiner, E. et al. (2012) Glia 60, 1646.
    Shipping and Ordering information