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Anti-Kir4.1 (KCNJ10) (extracellular) Antibody

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Cat #: APC-165
Alternative Name ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1 (Accession P49655). Extracellular loop.
Accession (Uniprot) Number P49655
Gene ID 29718
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/14 amino acid residues identical.
RRID AB_2341043.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity May have some cross-reactivity with rat Kir4.2 (Accession Q91ZF1).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1, 2. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:500).
    3, 4. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody, preincubated with Kir4.1/KCNJ10 (extracellular) Blocking Peptide (#BLP-PC165).
Immunohistochemistry

  • Rat brain sections (1:200), (Cui, Y. et al. (2018) Nature 554, 323.).

Live cell imaging / Immunocytochemistry
  • Expression of Kir4.1 in human U-87 MG cells
    Expression of Kir4.1 in human U-87 MG cells
    Cell surface detection of Kir4.1 in live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells using Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
References
  1. Takumi, T. et al. (1995) J. Biol. Chem. 270, 16339.
  2. Higashi, K. et al. (2001) Am. J. Physiol. 281, C922.
  3. Butt, A.M. and Kalsi, A. (2006) J. Cell Mol. Med. 10, 33.
Scientific background

Kir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.

Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.

Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.

The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. 

Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney.1,2

It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the K+ released during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 24/01/2021

Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize Kir4.1 potassium channel from rat, mouse, and human samples.

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For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse spinal cord lysate.
    Kelley, K.W. et al. (2018) Neuron 98, 306.
Immunohistochemistry citations
  1. Rat brain sections.
    Cui, Y. et al. (2018) Nature 554, 323.

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