Guinea pig Anti-Kir6.2 Antibody

Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3
    Cat #: AGP-067
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminus.
    Accession (Uniprot) Number P70673
    Gene ID 83535
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity The antibody is specific for Kir6.2 and does not cross-react with Kir6.1.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat heart membranes (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):
    1-2. Guinea pig Anti-Kir6.2 Antibody (#AGP-067), (1:500).
    3-4. Guinea pig Anti-Kir6.2 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human SHSY-5Y neuroblastoma cell lysate:
    1. Guinea pig Anti-Kir6.2 Antibody (#AGP-067), (1:200).
    2. Guinea pig Anti-Kir6.2 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Kir6.2 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-Kir6.2 Antibody (#AGP-067), (1:300), followed by goat-anti-guinea pig-Cy3 antibody (red staining). Kir6.2 staining appears in the hilus region in neuronal outlines (arrows). Nuclei are stained with DAPI (blue).
    References
    1. Babenko, A.P. et al. (1998) Annu. Rev. Physiol60, 667.
    2. Huopio, H. et al. (2002) Am. J. Physiol. 283, 207.
    Scientific background

    Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides.  

    The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle.  

    The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit.  

    The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and   SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. 

    Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Guinea pig Anti-Kir6.2 Antibody
    Immuno-colocalization of Kir6.2 and BDNF in mouse hippocampus.
    Immunohistochemical staining of immersion-fixed,free floating mouse brain frozen sections using Guinea pig Anti-Kir6.2 Antibody (#AGP-067), (1:300) and Anti-BDNF Antibody (#ANT-010), (1:300). A. Kir6.2 staining (red) appears in the dentate gyrus granule layer (G) and in hilar interneurons (arrows). B. BDNF staining (green) in the same section appears in the dentate gyrus granule layer (G) and in hilar interneurons (arrows). C. Merge of the two images reveals colocalization of Kir6.2 and BDNF in hippocampal interneurons.

    Last update: 25/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Kir6.2. Guinea pig Anti-Kir6.2 Antibody (#AGP-067) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir6.2 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir6.2 Antibody (#APC-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use