Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody

Potassium voltage-gated channel subfamily A member 3
    Cat #: AGP-005
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). 1st extracellular loop.
    Accession (Uniprot) Number P22001
    Gene ID 3738
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1-2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005), (1:200).
    3-4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of KV1.3 in mouse cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005), (1:100). KV1.3 staining (red) is detected in Bergmann glia outlines (arrow). DAPI is used as the counterstain (blue) .
    References
    1. Chandy, K.G. et al. (2001) Toxicon 39, 1269.
    2. Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
    Scientific background

    KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that KV1.3 function has centered a lot of attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.  

    Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/11/2018

    Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV1.3 potassium channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use
    Citations
    Immunohistochemistry citations
    1. Human head and neck tumor sections.
      Chimote, A.A. et al. (2017) Cancer Res. 77, 53.

    Related Products
      • Products for control experiments
        1. Anti-KV1.3 (KCNA3) Antibody (#APC-002). This antibody recognizes a different epitope and thus can also be used as a control for detecting KV1.3.
      • Antibodies for immuno-colocalization experiments
        1. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B). A biotin labeled primary antibody. It can be used in colocalization studies in conjunction with any of our antibodies raised in rabbit.