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Anti-KV1.5 (KCNA5) (extracellular)-FITC Antibody

Potassium voltage-gated channel subfamily A member 5

Cat #: APC-150-F
Alternative Name Potassium voltage-gated channel subfamily A member 5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DERELLRHPPVP(K), corresponding to amino acid residues 268-279 of rat KV1.5 (Accession P19024). 1st extracellular loop.
Accession (Uniprot) Number P19024
Gene ID 25470
Homology Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical; mouse - 9/13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APC-150), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of KV1.5 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of KV1.5 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-KV1.5 (KCNA5) (extracellular)-FITC Antibody (#APC-150-F), (2.5µg).
  • Cell surface detection of KV1.5 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of KV1.5 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-KV1.5 (KCNA5) (extracellular)-FITC Antibody (#APC-150-F), (5µg).
References
  1. Swanson, R. et al. (1990) Neuron 4, 929.
  2. Gutman, G.A. et al. (2005) Pharmacol. Rev. 57, 473.
  3. McGahon, M.K. et al. (2007) Am. J. Physiol. Heart Circ. Physiol. 292, H1001.
Scientific background

KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from the rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2

A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2

The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss-of-function mutation in the gene encoding the channel was found in atrial fibrillation patients, stressing its role as a cardiac action potential regulator.3

The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.

KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the “classical” non-selective potassium channel blockers.2

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 03/05/2022

Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize KV1.5 from mouse, rat and human samples.

Anti-KV1.5 (KCNA5) (extracellular)-FITC Antibody (#APC-150-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize KV1.5 from mouse, rat and human samples.

For research purposes only, not for human use
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