Overview
- Peptide GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1 (Accession Q63472). Intracellular, C-terminus.
Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4):1,2. Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200).
3,4. Anti-KCNH1 (EAG-1) Antibody, preincubated with KCNH1/EAG-1 Blocking Peptide (#BLP-PC104).- Mouse brain and colon lysates (Ousingsawat, J. et al. (2007) Clin. Cancer Res. 13, 824.).
- HEK 293T transfected cells (Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.).
- Paraffin embedded human gliomas (1:200) (Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.).
Expression of EAG1 in human MDA-468 mammary gland adenocarcinoma cells.Immunocytochemical staining of human MDA-468 mammary gland adenocarcinoma cells. A. Cells were stained with Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). C. Merged image of panels A and B.
- Ludwig, J. et al. (1994) EMBO J. 13, 4451.
- Bauer, C.K. et al. (2001) J. Membrane Biol. 182, 1.
- Pardo, L.A. et al. (1999) EMBO J. 18, 5540.
KV10.1 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N- and C-termini.
The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.
Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat KV10.1 channel. Anti-KCNH1 (EAG-1) Antibody (#APC-104) can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV10.1 from rat, human, and mouse samples. The antibody is specific for KV10.1 and will not cross-react with KV10.2.
Applications
Citations
Powered by Bioz- HEK-293T transfected cells.
Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.
- HEK-293T transfected cells.
Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.
- Human breast cancer tissue sections.
Peretti, M. et al. (2019) Sci. Rep. 9, 1175.
- Human MCF-7 cells.
Peretti, M. et al. (2019) Sci. Rep. 9, 1175.
- Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.
- Spitzner, M. et al. (2008) J. Biol. Chem. 283, 7421.
- Ousingsawat, J. et al. (2007) Clin. Cancer Res. 13, 824.
- Spitzner, M. et al. (2007) FASEB J. 21, 35.
- Cherubini, A. et al. (2005) Mol. Biol. Cell 16, 2972.
