- Peptide CRLNQEISRLNQEVS, corresponding to amino acids 881-895 of rat KCNH4 (Accession Q9R1T9). Intracellular, C-terminal part.
- Rat brain and cerebellum lysate (1:400).
- Western blot analysis of rat brain (lanes 1 and 3) and cerebellum (lanes 2 and 4) lysates:1,2. Anti-KV12.3 (KCNH4) Antibody (#APC-116), (1:200).
3,4. Anti-KV12.3 (KCNH4) Antibody, preincubated with Kv12.3/KCNH4 Blocking Peptide (#BLP-PC116).
- Rat testis sections (paraffin-embedded), (1:50).
KV12.3 (also known as KCNH4, Elk3) is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. These are the eag (KV10) with two members, the eag-related channels (erg or KV11) and the eag-like K+ channels (elk or KV12) with three members each.
As with all members of the family, KV12.3 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C- termini. The functional channel is a tetramer composed of four subunits. It has been suggested that the KV12 subfamily members can form functional heteromultimers within the subfamily.1,2
KV12.3 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as the testis and lung.3
The physiological function of the KV12.3 channel is yet to be established, although a role in the modulation of overall excitability of neurons has been suggested.3
Currently, there isn’t much pharmacological data concerning the modulation of the KV12.3 channel except for blocking of the channel by Ba2+.3