Guinea pig Anti-KV2.1 Antibody

KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1
    Cat #: AGP-109
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1 (Accession P15387). Intracellular, C-terminus.
    Accession (Uniprot) Number P15387
    Gene ID 25736
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 15/17 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Guinea pig Anti-KV2.1 Antibody (#AGP-109), (1:500).
    3,4. Guinea pig Anti-KV2.1 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of KV2.1 channel in rat hippocampus
    Immunohistochemical staining of rat hippocampal CA1 region using Guinea pig Anti-KV2.1 Antibody (#AGP-109), (1:400). A. KV2.1 staining (green) appears in the pyramidal layer (P) and in stratum oriens (SO) interneurons (horizontal arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged image of panels A and B.
    References
    1. Wulff, H. et al. (2009) Nat. Rev. Drug Discov. 8, 982.
    2. Thiffault, I. et al. (2015) J. Gen. Physiol. 146, 399.
    Scientific background

    Voltage gated K+ channels are involved in diverse physiological processes ranging from repolarization of neuronal and cardiac action potential, regulation of Ca2+ signaling and cell volume to driving cellular proliferation and migration.

    KV2.1 consists of four α-subunits, each containing six transmembrane α-helical segments, S1-S6, and a membrane-re-entering P-loop, which are arranged circumferentially around a central pore. This ion conduction pore is lined by four S5-P-S6 sequences. The four S1–S4 segments, each containing four positively charged arginine residues in the S4 helix, act as voltage sensor domains and “gate” the pore by “pulling” on the S4–S5 linker.

    KV2.1, encoded by the KCNB1 gene, is a classical delayed rectifier channel that is involved in neuronal repolarization. Its function can be diversified through heteromultimerization with “silent” KV units, which modify inactivation, trafficking, drug sensitivity and expression.

    Inhibition of KV2.1 in pancreatic β-cells enhances insulin secretion, suggesting a potential therapeutic strategy for type 2 diabetes. This effect is presumed to occur through non-conducting functions - namely, a physical interaction with syntaxin (a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex) that facilitates vesicle fusion1.

    Recently, a “de novo” pathogenic KV2.1 variant was discovered in a family suffering from idiopathic epileptic encephalopathy. This variant is a missense mutation in the coding region of the KCNB1 gene. Expression of the V378A variant results in voltage-activated currents that are sensitive to the selective KV2 channel blocker guangxitoxin-1E. Currents through the mutant channel are non-selective among monovalent cations2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Guinea pig Anti-Kv2.1 AntibodyImmuno-colocalization of Slitrk1 and KV2.1 in rat parietal cortex.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-Slitrk1 extracellular) Antibody (#ASR-081), (1:200) and Guinea pig Anti-KV2.1 Antibody (#AGP-109), (1:200). A. Sltrk1 staining (red) appears in profiles of pyramidal neurons. B. KV2.1 staining (green) is detected in profiles of pyramidal neurons. C. Merge of the two images shows colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).

    Last update: 11/12/2018

    Guinea pig Anti-KV2.1 Antibody (#AGP-109) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV2.1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV2.1 Antibody (#APC-012) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use