Overview
- Peptide (C)RLRRLQNTNSASERE, corresponding to amino acid residues 472 - 486 of mouse KCNG4 (Accession Q80XM3). Intracellular, C-terminus.
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-Kv6.4 (KCNG4) Antibody (#APC-138), (1:200).
3-4. Anti-Kv6.4 (KCNG4) Antibody, preincubated with Kv6.4 (KCNG4) Blocking Peptide (BLP-PC138).
Expression of Kv6.4 in rat cerebral aqueduct.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-Kv6.4 (KCNG4) Antibody (#APC-138), (1:600), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Kv6.4 immunoreactivity (red) appears in the wall of the aqueduct (arrow). B. Pre-incubation of the antibody with Kv6.4 (KCNG4) Blocking Peptide (BLP-PC138), suppressed staining. Cell nuclei are stained with DAPI (blue).
- De Lera Ruiz, M. et al. (2015) J. Med. Chem., 58, 7093.
- Bocksteins, E. et al. (2016) J. Gen. Physiol., 147, 105.
- Möller, L. et al. (2020) Proc. Natl. Acad. Sci. U.S.A., 117, 9365.
Voltage-gated K+ (Kv) channels are potassium selective multimeric channels that change their conformation in response to changes in the membrane potential. Kv channels play a central role in the determination of cellular excitability due to their contribution to the shape, duration and frequency of action potentials. A wide range of physiological activities are regulated via KV actions including cell signaling, proliferation and migration1.
Kv channels are divided into several subfamilies based in sequence homology. Members of the Kv5, Kv6, Kv8, and Kv9 subfamilies, are unable to form functional channels, even though they have the typical topology of a Kv α-subunit and have therefore been designated silent Kv (KvS) subunits.
KV6.4 (also known as KCNG4) is part of the electrically silent KV6 subfamily that includes four members Kv6.1-Kv6.42. Members of the Kv6 subfamily selectively interact with members of the Kv2 subfamily, forming functional Kv2/KvS heterotetramers that possess unique biophysical and pharmacological properties 2.
Heterotetramerization of KV6.4 with Kv2 results in the formation of functional channels. The stoichiometry of Kv2/Kv6 complex was recently found to be 2:2 stoichiometry with activation V1/2 of 5.7 ± 3.1 mV and inactivation V1/2 of -62.8 ± 1.6 mV3.
Anti-Kv6.4 (KCNG4) Antibody (#APC-138) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kv6.4 from mouse and rat samples. The antibody will not recognize Kv6.4 from human samples.
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