Anti-LAT2 (SLC7A8) (extracellular) Antibody

Large Neutral Amino Acids Transporter Small Subunit 2, L-Type Amino Acid Transporter 2, Solute carrier family 7 member 8

Cat #: ANT-108
Alternative Name Large Neutral Amino Acids Transporter Small Subunit 2, L-Type Amino Acid Transporter 2, Solute carrier family 7 member 8
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CKGEFFWLEPKNAFEN, corresponding to amino acid residues 211 - 226 of rat SLC7A8 (Accession Q9WVR6). Extracellular, 3rd loop.
Accession (Uniprot) Number Q9WVR6
Gene ID 84551
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; Human – 15 out of 16 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4):
    Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4):
    1-2. Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200).
    3-4. Anti-LAT2 (SLC7A8)(extracellular) Antibody, preincubated with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108).
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 4), human MDA-MB-231 breast adenocarcinoma cell line lysates (lanes 2 and 5) and human MCF-7 breast adenocarcinoma cell line lysates (lanes 3 and 6):
    Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 4), human MDA-MB-231 breast adenocarcinoma cell line lysates (lanes 2 and 5) and human MCF-7 breast adenocarcinoma cell line lysates (lanes 3 and 6):
    1-3. Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200).
    4-6. Anti-LAT2 (SLC7A8)(extracellular) Antibody, preincubated with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108).
Immunohistochemistry
  • Expression of SLC7A8 in mouse hippocampus.
    Expression of SLC7A8 in mouse hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity of SLC7A8 (green) in the hilus (H). B. Pre-incubation of the antibody with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of SLC7A8 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
    Cell surface detection of SLC7A8 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (5μg) + goat-anti-rabbit-FITC.
References
  1. Wang et al (2015) Am J Cancer Res, 15, 1281.
  2. Krause et al (2017) Mol Cell Endocrinol 15, 68.
  3. Del Amo et al (2008) J Pharmacol Sci 119, 368.
  4. Espino Guarch et al (2018) eLife 7, e31511.
  5. Knöpfel EB et al (2019) Front. Physiol. 10, 688.
Scientific background

L-type amino acid transporter (LAT) family are transporters responsible for the uptake of neutral amino acids into cells. The LATs family contain four different members LAT1 (SLC7A5), LAT2 (SLC7A8), LAT3 (SLC43A1) and LAT4 (SLC43A2). LATs transporters are known to carried out their function in an Na+ and pH independent manner1. In recent years, LATs family shown to participate in the uptake of thyroid hormones (THs) and their derivatives2.

LAT2 (SLC7A8) is a transmembrane protein first discovered back in 1999 using sequence similarity to LAT1. According to the predicted membrane topology, LAT2 consists of 12 transmembrane domains (TMDs) and N- and C- termini located in the cytosol. LAT2 associate with the 4F2hc (4F2 antigen heavy chain; CD98 heavy chain) glycoprotein to form a dimer that act as a neutral amino acid transporter3.

LAT2 is expressed in various tissues, including the intestinal wall, blood–brain barrier, and kidney. Mutations in LAT2 protein cause age-related hearing loss in mice and humans5. In addition, deletion LAT2 in mice led to increased incidence of cataract4,5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 14/06/2021

Anti-LAT2 (SLC7A8) (extracellular) Antibody (#ANT-108) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize SLC7A8 from rat, mouse and human samples.

For research purposes only, not for human use
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