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Anti-LAT3/SLC43A1 (extracellular) Antibody

Large neutral amino acids transporter small subunit 3, L-type amino acid transporter 3, Solute carrier family 43 member 1
Cat #: ANT-193
Alternative Name Large neutral amino acids transporter small subunit 3, L-type amino acid transporter 3, Solute carrier family 43 member 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GKERETNEQRQKVE, corresponding to amino acid residues 336-349 of mouse LAT3 (SLC43A1) (Accession Q8BSM7). 4th extracellular loop.
Accession (Uniprot) Number Q8BSM7
Gene ID 72401
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 13/14 amino acid residues identical; human – 10/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates:
    Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates:
    1, 2. Anti-LAT3/SLC43A1 (extracellular) Antibody (#ANT-193), (1:400).
    3, 4. Anti-LAT3 (SLC43A1) (extracellular) Antibody, preincubated with LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193).
  • Western blot analysis of human chronic myelogenous leukemia K562 (lanes 1 and 3) and human prostate carcinoma LN-CaP (lanes 2 and 4) cell lines lysates:
    Western blot analysis of human chronic myelogenous leukemia K562 (lanes 1 and 3) and human prostate carcinoma LN-CaP (lanes 2 and 4) cell lines lysates:
    1, 2. Anti-LAT3 (SLC43A1) (extracellular) Antibody (#ANT-193), (1:200).
    3, 4. Anti-LAT3 (SLC43A1) (extracellular) Antibody, preincubated with LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193).
References
  1. Christensen, H.N. (1990) Physiol. Rev. 70, 43.
  2. Babu, E. et al. (2003) J. Biol. Chem. 278, 43838.
  3. Sekine, T. et al. (2000) Pflugers Arch. 440, 337.
  4. Zhang, B.K. et al. (2019) Cell Commun. Signal. 17, 83.
Scientific background

LAT3, (L-type amino acid transporter 3) is an amino acid transporter that belongs to the system L family of membrane transporters.

The system L transporter family consists of various transporter subtypes with different characteristics in substrate selectivity and transport properties. It is characterized by a Na+ independent transport and its inhibition by 2-aminobicyclo heptane-2-carboxylicacid (BCH).

System L transporters are a major route to provide cells with branched-chain and aromatic amino acids. Transporters that belong to this family are present in the basolateral membrane of epithelial cells and play important roles in the absorption of amino acids through the epithelial cells of the small intestine and renal proximal tubules. They are essential in the permeation of amino acids through the blood–tissue barriers such as blood brain barrier and placenta barrier1.

LAT3 consists of seven transmembrane domains with a relatively long extracellular loop with predicted N-linked glycosylation sites between transmembrane domains 1 and 2 and a long intracellular loop with several putative phosphorylation sites between transmembrane domains 6 and 72,3.

LAT3 was shown to be up-regulated in prostate cancer and responsible for the increased uptake of leucine into the tumor cells. Hence, it is an appealing target for cancer therapy4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 23/08/2020

Anti-LAT3/SLC43A1 (extracellular) Antibody (#ANT-193) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC43A1 from human, rat, and mouse samples.

For research purposes only, not for human use
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