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Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody

Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2
Cat #: ALR-021
Alternative Name Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CEPREVRRVQWPATQ(G), corresponding to amino acid residues 480-494 of rat Latrophilin-1 (Accession O88917). Extracellular, N-terminus.
Accession (Uniprot) Number O88917
Gene ID 65096
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_11219339.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (1:200).
    3,4. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody, preincubated with Latrophilin-1/LPHN1 (extracellular) Blocking Peptide (#BLP-LR021).
Immunohistochemistry
  • Expression of Latrophilin-1 receptor in rat hippocampus
    Expression of Latrophilin-1 receptor in rat hippocampus
    Immunohistochemical staining of rat hippocampal dentate granule layer (G) using Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021). A. Latrophilin-1 receptor staining (red) appears in astrocytes. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Live cell imaging / Immunocytochemistry
  • Expression of Latrophilin-1 receptor in SH-SY5Y cell line
    Expression of Latrophilin-1 receptor in SH-SY5Y cell line
    Cell surface detection of Latrophilin-1 receptor in intact living human neuroblastoma (SH-SY5Y) cells. A. Extracellular staining of cells with Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI is used as the counterstain (blue). C. Merged images of A and B.
References
  1. Sugita, S. et al. (1998) J. Biol. Chem. 273, 32715.
  2. Martinez, A.F. et al. (2011) Am. J. Med. Genet. Part B 156, 1.
  3. Matsushita, H. et al. (1999) FEBS Lett. 443, 348.
  4. Davletov, B.A. et al. (1996) J. Biol. Chem. 271, 23239.
  5. Capogna, M. et al. (2003) J. Neurosci. 23, 4044.
  6. Lelyanova, V.G. et al. (2009) Bull. Exp. Biol. Med. 147, 701.
  7. Silva, J.P. et al. (2011) Proc. Natl. Acad. Sci. U.S.A. 108, 12113.
  8. Levine, A. et al. (1994) Cell 77, 587.
  9. Tucker, R.P. and Chiquet-Ehrismann, R. (2006) Dev. Biol. 290, 237.
  10. Ichtchenko, K. et al. (1999) J. Biol. Chem. 274, 5491.
Scientific background

Latrophilins (Latrophilin1-3) are members of the adhesion G-protein coupled receptor subfamily. Like all GPCRs, Latrophilins have seven transmembrane domains and are distinguished by a large extracellular N-terminal tail and a large intracellular C-terminal tail1. The N-terminus has several cell adhesion domains and undergoes proteolysis after synthesis, while the C-terminal has various consensus post-translational sites like phosphorylation and palmitoylation2. In addition, Latrophilins undergo alternative splicing3.

Latrophilin-1 was discovered by its ability to bind α-Latrotoxin (α-LTX), a toxin isolated from the black widow spider venom4. α-LTX induces exocytosis by creating a Ca2+ influx in the presynaptic membrane. α-LTX can also stimulate small vesicle exocytosis in a Ca2+ independent manner. Three receptors have been found to bind α-LTX. Of the three, Latrophilins are responsible for the Ca2+-independent effects of α-LTX2. The binding of α-LTX to Latrophilin-1 increases exocytosis of neurotransmitters5,6.

In an attempt to find the natural ligand of Latrophilin-1, Lasso, a splice variant of Teneneurin-2 was discovered to be an endogenous binding partner of the adhesion-GPCR7. Teneneurins are large glycoproteins with a single transmembrane domain8,9. Like Latrophilin-1, Teneneurins are mostly expressed in the brain where they modulate neurite outgrowth, axon guidance and synaptogenesis7.

Regarding the localization of Latrophilins, Latrophilin-1 is expressed predominantly in the brain, Latrophilin-2 is highly expressed in the liver and lung, while Latrophilin-3 is almost exclusively detected in the brain1,10.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Latrophilin-1 from rat, human, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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