Overview
- Peptide (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor (Accession P61794). Extracellular, N-terminus.
Western blot analysis of rat brain membrane (lanes 1 and 3), mouse brain membrane (lanes 2 and 4) and human brain glioblastoma U87 MG cell line (lanes 5 and 6) lysate:1,2,5. Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (1:400).
3,4,6. Anti-LPAR1 (EDG2) (extracellular) Antibody, preincubated with LPAR1/EDG2 (extracellular) Blocking Peptide (#BLP-LR031).
Expression of LPAR1 in rat corpus callosum (CC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. LPAR1 immunoreactivity (red) appears in CC cells with oligodendrocyte morphology (arrows). B. Pre-incubation of the antibody with LPAR1 (EDG2) (extracellular) Blocking Peptide (#BLP-LR031), suppressed staining. Cell nuclei are stained with DAPI (blue).
Cell surface detection of LPAR1 (EDG2) by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (5μg) + goat-anti-rabbit-FITC.
- Contos, J.J.A. et al. (2000) Mol. Pharmacol. 58, 1188.
- Gohla, A. et al. (1998) J. Biol. Chem. 273, 4653.
- Boucharaba, A. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 9643.
Lysophosphatidic acid is a naturally occurring lysophospholipid that activates diverse cellular actions in various cells including proliferation and morphological changes. There are currently three known genes that encode proteins of the LPA receptor family- Edg2, Edg4 and Edg7.
Lysophosphatidic acid receptor 1 (also known as Lpa1), encoded by Edg2, belongs to G-coupled protein receptor (GPCR) superfamily. All three proteins share 50-54% identical amino-acids. Lpa1 mostly activates the Gi pathway that leads to inhibition of adenylate cyclase and thus inhibition of cAMP production. Through this pathway, Lpa1 also initiates the Ras/MAP kinase cascade which is primarily responsible for increased cell proliferation1. Interestingly, binding of lysophosphatidic acid to the G13 G-protein activates the small GTP-binding protein Rho and induces the rapid remodeling of the actin cytoskeleton2.
Lpa1 has been implicated in the pathogenesis of metastatic bone cancer. Silencing of Lpa1 expression in breast and ovarian cancer cells significantly alters the progression of bone metastasis through reduction of tumor burden and inhibition of bone resorption. A specific Lpa1 antagonist exhibits specificity for tumor cell-platelet interaction without abrogating normal platelet function3.
Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031) is a highly specific antibody directed against an epitope of the rat LPA1 receptor. The antibody can be used in western blot analysis. It has been designed against an extracellular domain and thus is highly suitable for detecting the receptor in living cells. It has been designed to recognize LPA1 receptor from human, rat, and mouse samples.
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