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Anti-LRRC26 Antibody

Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC

Cat #: APC-070
Alternative Name Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)RRTTVRHLIRRQPD, corresponding to amino acid residues 301-314 of rat LRRC26 (Accession Q6P7C4). Intracellular, C-terminus.
Accession (Uniprot) Number Q6P7C4
Gene ID 311803
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 12/14 amino acid residues identical.
RRID AB_2756741.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Won’t recognize human LRRC26.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse brain membranes:
    Western blot analysis of mouse brain membranes:
    1. Anti-LRRC26 Antibody (#APC-070), (1:400).
    2. Anti-LRRC26 Antibody, preincubated with LRRC26 Blocking Peptide (#BLP-PC070).
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-LRRC26 Antibody (#APC-070), (1:200).
    2. Anti-LRRC26 Antibody, preincubated with LRRC26 Blocking Peptide (#BLP-PC070).
References
  1. Braun, A.P. (2010) Channels 4, 249.
  2. Evanson, K.W. et al. (2014) Circ. Res. 115, 423.
Scientific background

BK (KCa1.1) channels are critically involved in diverse physiological processes in both electrically excitable and non-excitable cells.

Leucine-rich repeat containing protein 26 (also known as BK channel auxiliary gamma subunit or LRRC26) is a single spanning transmembrane protein of ~37 kDa. LRRC26 protein contains five major leucine rich repeats in its extracellular domain which are all required for modulating BK channel activity. The single transmembrane segment of LRRC26 is required for co-assembly with the BKα subunit complex.

When LRRC26 is acutely knocked down in LNCaP cells, the voltage-dependent activation of endogenous BK current shifts from a low to a high V1/2 value, whereas recombinant expression of LRRC26 cDNA in naïve cells (such as PC3 prostate cancer cells and HEK 293 cells) normally lacking LRRC26 shifts BK channel activation from high to low V1/2 values, reproducing the channel behavior observed in LNCaP cells. This findings indicate that LRRC26 is sufficient to impart low voltage gating behavior to wild-type pore-forming, BKα subunits.

LRRC26 lowers the energy barrier for activation of BK channels, and is able to stabilize channel opening in response to voltage, thereby maintaining channels in the open state to a much greater extent at any given membrane potential1.

LRRC26 is expressed in cerebral artery myocytes where it is primarily plasma membrane localized and associated with BKα subunits. LRRC26 serves as an arterial myocyte BK channel auxiliary γ subunit that elevates voltage and apparent Ca2+ sensitivity to induce vasodilation2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Anti-LRRC26 Antibody (#APC-070) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LRRC26 from rat and mouse samples. It does not recognize the protein from human samples.

For research purposes only, not for human use
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