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Anti-MAGT1 (extracellular) Antibody

Magnesium Transporter 1Oligosaccharyl Transferase Subunit MAGT1Implantation-Associated Protein, SLC58A1

Cat #: ANT-197
Alternative Name Magnesium Transporter 1Oligosaccharyl Transferase Subunit MAGT1Implantation-Associated Protein, SLC58A1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SAQRKKEMVLSEK, corresponding to amino acid residues 28-40 of human MAGT1 (Accession Q9H0U3). Extracellular, N-terminus.
Accession (Uniprot) Number Q9H0U3
Gene ID 84061
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical. Rat 11 out of 13 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The immunizing peptide bears some homology (7 out of 13 amino acid residues) with the related protein TUSC3 (Accession Q13454).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse colon lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
    Western blot analysis of mouse colon lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
    1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
    4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
  • Western blot analysis of human HepG2 hepatocarcinoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
    Western blot analysis of human HepG2 hepatocarcinoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human
    THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
    1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
    4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
Immunohistochemistry
  • Expression of MAGT1 in rat cortex.
    Expression of MAGT1 in rat cortex.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the parietal cortex region showed MAGT1 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with MAGT1 (extracellular) Blocking Peptide (BLP-NT197), suppressed staining. Cell nuclei are stained with DAPI (blue).
  • Expression of MAGT1 in rat supra-optic nucleus.
    Expression of MAGT1 in rat supra-optic nucleus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. MAGT1 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with MAGT1 (extracellular) Blocking Peptide (BLP-NT197), suppressed staining. Cell nuclei are stained with DAPI (blue). OT = optic tract.
Indirect flow cytometry
  • Cell surface detection of MAGT1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of MAGT1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-MAGT1 (extracellular) Antibody (#ANT-197), (5μg) + goat-anti-rabbit-FITC.
References
  1. Trapani et al. (2015), Magnes Res, 28, 46.
  2. Blommaert et al. (2019), Proc Natl Acad Sci USA, 116, 9865.
  3. Matsuda-Lennikov et al. (2019), J Biol Chem, 294, 13638.
Scientific background

Magnesium Transporter 1 (MAGT1) is a 335 amino acid peptide that is a part of the MAGT group of magnesium transporters. Magnesium (Mg2+) plays an essential cellular role, as a cofactor for ATP, nucleic acids, and numerous enzymes. It has been reported that it serves as a second messenger in intracellular signaling, and hence magnesium levels maintenance has a vast effect on cellular steady state 1

In addition to its Mg2+ transporter activity, MAGT1 was also found to be a subunit of the oligosaccharyltransferase (OST) complex, through which it is responsible for protein glycosylation 2,3.

MAGT1 was shown to be related to the development of XMEN (X-linked immunodeficiency with magnesium defect, Epstein Barr Virus infection, and neoplasia) syndrome, for which Mg2+ supplementation has been shown to be beneficial. It is also related to atypical ATRX syndrome, intellectual disability and skin abnormalities.

Understanding the molecular basis of the involvement of magnesium and its channels in human pathogenesis can lead to the development of new medications for many diseases.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-MAGT1 (extracellular) Antibody (#ANT-197) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MAGT1 from mouse, rat and human samples.

For research purposes only, not for human use
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