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Anti-SLC47A1 Antibody

MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein

Cat #: ANT-131
Alternative Name MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)ESHGEIMMTDLEKK, corresponding to amino acid residues 496-509 of mouse SLC47A1 (Accession Q8K0H1). 5th intracellular loop.
Accession (Uniprot) Number Q8K0H1
Gene ID 67473
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human - no apparent homology.
RRID AB_2756729.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize human SLC47A1.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6):
    Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6):
    1-3. Anti-SLC47A1 Antibody (#ANT-131), (1:200).
    4-6. Anti-SLC47A1 Antibody, preincubated with SLC47A1 Blocking Peptide (#BLP-NT131).
References
  1. Lee, J.H. et al. (2014) J. Agric. Food Chem. 62, 9690.
  2. Wright, S.H. et al. (1995) Pflugers Arch. Eur. J .Physiol. 429, 313.
  3. Jong N.N. and McKeage, M.J. (2014) Biopharm. Drug Dispos. 35, 450.
  4. Maron, L.G. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 5241.
  5. Wittwer, M.B. et al. (2013) J. Med. Chem. 56, 781.
Scientific background

MATE1 (SLC47A1) and MATE2 (SLC47A2) are multidrug and toxin extrusion transporter 1 and 2 function as cationic pumps in the kidneys, liver, and intestine1. Both MATEs excrete molecules with a positive net-charge from renal tubular epithelial cells in the kidney (where they are co-expressed with organic anion- and cation-transporters; OATs and OCTs), and into the urine. In the liver, MATE1, but not MATE2, regulates the transportation of tetraethylammonium (TEA) and 1-methyl-4-phenylpyridinium (MPP) – again, by cation-powered pumps2.

Fairly recent X-ray crystallography has revealed that mammalian MATE1 has 13 transmembrane helices and a long cytoplasmic tail. Cysteine and histidine residues are responsible for the protein's functionality1.

Overexpression of MATE1 is helpful in the delivery of metal-based anti-cancer drugs, such as cisplatin and oxaliplatin, as it effectively accumulates platinum intracellularly3. Furthermore, a rare increase in copy number of MATE1 in maize protects the plant from aluminum (Al) toxicity4. On the other hand, inhibition of MATE1 may lead to potentially dangerous drug-drug interactions, due to damaged drug extrusion regulation5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Multidrug and toxin extrusion protein 1 (MATE1, SLC47A1). Anti-SLC47A1 Antibody (#ANT-131) can be used in western blot analysis. It has been designed to recognize SLC47A1 from rat and mouse samples. The antibody will not recognize SLC47A1 from human samples.

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For research purposes only, not for human use

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