- Peptide (C)ESHGEIMMTDLEKK, corresponding to amino acid residues 496-509 of mouse SLC47A1 (Accession Q8K0H1). 5th intracellular loop.
- Rat kidney, rat testis and mouse brain lysates (1:200-1:1000).
- Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6):1-3. Anti-SLC47A1 Antibody (#ANT-131), (1:200).
4-6. Anti-SLC47A1 Antibody, preincubated with SLC47A1 Blocking Peptide (#BLP-NT131).
MATE1 (SLC47A1) and MATE2 (SLC47A2) are multidrug and toxin extrusion transporter 1 and 2 function as cationic pumps in the kidneys, liver, and intestine1. Both MATEs excrete molecules with a positive net-charge from renal tubular epithelial cells in the kidney (where they are co-expressed with organic anion- and cation-transporters; OATs and OCTs), and into the urine. In the liver, MATE1, but not MATE2, regulates the transportation of tetraethylammonium (TEA) and 1-methyl-4-phenylpyridinium (MPP) – again, by cation-powered pumps2.
Fairly recent X-ray crystallography has revealed that mammalian MATE1 has 13 transmembrane helices and a long cytoplasmic tail. Cysteine and histidine residues are responsible for the protein's functionality1.
Overexpression of MATE1 is helpful in the delivery of metal-based anti-cancer drugs, such as cisplatin and oxaliplatin, as it effectively accumulates platinum intracellularly3. Furthermore, a rare increase in copy number of MATE1 in maize protects the plant from aluminum (Al) toxicity4. On the other hand, inhibition of MATE1 may lead to potentially dangerous drug-drug interactions, due to damaged drug extrusion regulation5.