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Anti-SLC47A2 Antibody

MATE2, Multidrug and toxin extrusion protein 2, H+/organic cation antiporter kidney-specific
Cat #: ANT-132
Alternative Name MATE2, Multidrug and toxin extrusion protein 2, H+/organic cation antiporter kidney-specific
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m
May also work in: r*
Immunogen
  • Peptide (C)RAGLKSTKELIPTPAD, corresponding to amino acid residues 481-496 of mouse SLC47A2 (Accession Q3V050). 6th intracellular loop.
Accession (Uniprot) Number Q3V050
Gene ID 380701
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 14/16 amino acid residues identical; human – not recommended for use with human samples.
RRID AB_2756730.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse kidney (lanes 1 and 3) and mouse testis (lanes 2 and 4) lysates:
    Western blot analysis of mouse kidney (lanes 1 and 3) and mouse testis (lanes 2 and 4) lysates:
    1,2. Anti-SLC47A2 Antibody (#ANT-132), (1:200).
    3,4. Anti-SLC47A2 Antibody, preincubated with SLC47A2 Blocking Peptide (#BLP-NT132).
References
  1. Hediger, M.A. et al. (2013) Mol. Aspects Med. 34, 95.
  2. Sato, T. et al. (2008) Biochem. Pharmacol. 76, 894.
  3. Motohashi, H. and Inui, K. (2013) Mol. Aspects Med. 34, 661.
  4. Kusuhara, H. et al. (2011) Clin. Pharmacol. Ther. 89, 837.
  5. Staud, F. et al. (2013) Int. J. Biochem. Cell Biol. 45, 2007.
Scientific background

Solute carriers control the efflux and uptake of crucial compounds such as sugars, amino acids and ions in cells and organelles1Multidrug and toxin extrusion proteins, also known as MATEs are responsible for the efflux of organic cations, endogenous compounds and drugs from cells. The driving force for this transport is derived from an oppositely directed proton gradient thus making MATE transporters secondary active transporters. MATEs work in concert with OCTs (Organic cation transporters). While the latter are located in the basolateral cell membrane of proximal tubules cells MATEs are expressed on the apical membrane2. MATEs can also transport zwitterions and anionic compounds in addition to cations.

MATE2 carrier is part of the SLC47 family encoded by the SLC47A2 gene, predominantly expressed in apical brush border membranes of the proximal tubules in kidneys. It exhibits proton gradient dependent secretion of cationic drugs across the membrane. It is predicted to have 12 transmembrane helices similar to other MATE family members but also an additional COOH-terminal helix. Histidine and Cysteine residues in positions 382, 59 and 123 respectably are required for MATE2 proper functioning3.

Metformin, a commonly used hypoglycemic agent and platinum based chemotherapeutics are mainly excreted by MATE transporters in the kidney. Changes in the expression of MATEs can dramatically influence the concentration of these substances in the body and cause serious side effects. In a recent study, pyrimethamine, an inhibitor of MATE transporters reduced metformin renal clearance by 35%4. Inhibition of MATE transporters by anti-neoplastic drugs can play a role in chemosensitivity of cancerous cells. MATEs are a potential target for prevention of resistance to treatment and toxicity during chemotherapy5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Multidrug and toxin extrusion protein 2 (MATE2, SLC47A2). Anti-SLC47A2 Antibody (#ANT-132) can be used in western blot analysis. It has been designed to recognize SLC47A2 from rat and mouse samples. The antibody will not recognize SLC47A2 from human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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