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Anti-MEGF10 (extracellular) Antibody

Multiple EGF Like Domains 10, Multiple EGF-Like Domains Protein 10

Cat #: ANR-205
Alternative Name Multiple EGF Like Domains 10, Multiple EGF-Like Domains Protein 10
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CEDR(S)EQGTYGND, corresponding to amino acid residues 178 - 190 of mouse MEGF10 (Accession Q6DIB5). Extracellular, N-terminus.
Accession (Uniprot) Number Q6DIB5
Gene ID 70417
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat, human – 12 out of 13 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity This antibody is specific for MEGF10, it won't recognize the closely related protein MEGF11 (Accession Q80T91)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
MEGF10 (extracellular) Blocking Peptide (#BLP-NR205)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-MEGF10 (extracellular) Antibody (#ANR-205), (1:400).
    3-4. Anti-MEGF10 (extracellular) Antibody, preincubated with MEGF10 (extracellular) Blocking Peptide (BLP-NR205).
  • Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 4), human MCF-7 breast adenocarcinoma (lanes 2 and 5) and human 1311N1 astrocytoma cell line lysate (lanes 3 and 6):
    Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 4), human MCF-7 breast adenocarcinoma (lanes 2 and 5) and human 1311N1 astrocytoma cell line lysate (lanes 3 and 6):
    1-3. Anti-MEGF10 (extracellular) Antibody (#ANR-205), (1:400).
    4-6. Anti-MEGF10 (extracellular) Antibody, preincubated with MEGF10 (extracellular) Blocking Peptide (BLP-NR205).
Immunohistochemistry
  • Expression of MEGF10 in rat substantia nigra.
    Expression of MEGF10 in rat substantia nigra.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MEGF10 (extracellular) Antibody (#ANR-205), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. MEGF10 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with MEGF10 (extracellular) Blocking Peptide (BLP-NR205), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of MEGF10 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of MEGF10 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-MEGF10 (extracellular) Antibody (#ANR-205), (5μg) + goat-anti-rabbit-FITC.
References
  1. Fujita, Y. et al. (2017) Neurosci. Lett. 653, 25.
  2. Iram, T. et al. (2016) J. Neurosci. 36, 19.
  3. Konishi, H. et al. (2022) Glia. 70, 1009.
  4. Singh, Thoudam Debraj et al. (2010) FEBS Lett. 584, 3936.
  5. Saha, Madhurima et al. (2017) Hum. Mol. Genet. vol. 26, 2984.
  6. Logan, C.V. et al. (2017) Nat. Genet. 43, 1189.
Scientific background

MEGF10 is a member of the multiple epidermal growth factor-like domains protein family. MEGF10 is a type I transmembrane protein that consists of 15 EGF-like domains in its extracellular region and is the mammalian homolog of Draper (a Drosophila phagocytosis apoptotic cell receptor)1.

MEGF10 is a mediator for apoptotic cell phagocytosis by central nervous system (CNS) macrophages- microglia and astrocytes, through binding with high affinity to C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells2. Also necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum2.  In this regard, MEGF10 works alongside other phagocytic receptors expressed in astrocytes or microglia such as the tyrosine kinase receptors MERTK and AXL, the G-protein coupled receptors Bai1 and GPR56 or the TREM2 receptor3.

MEGF10 is involved in the uptake of amyloid-β peptide (Aβ42) in the brain. Alzheimer's disease (AD) is characterized by extracellular accumulation of senile plaques, the major component of which is the amyloid-β peptide (Aβ), and the presence of neurofibrillary tangles. MEGF10 as a functional receptor that mediates the uptake of amyloid-β peptide will help elucidate the molecular mechanisms of amlyoid-β clearance in Alzheimer's disease1,4.

MEGF10 is also an essential factor in the regulation of myogenesis. It controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway5. In fact, mutations in the MEGF10 gene are the underlying cause of Early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD), a lethal congenital myopathy characterized by hypotonia with respiratory distress 5,6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/01/2023

Anti-MEGF10 (extracellular) Antibody (#ANR-205) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MEGF10 from mouse, rat and human samples.

For research purposes only, not for human use
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