Anti-MC4 Receptor (extracellular) Antibody

Melanocortin receptor 4, MC4R, MC4-R
    Cat #: AMR-024
    Alternative Name Melanocortin receptor 4, MC4R, MC4-R
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R (Accession P32245). Extracellular, N terminal.
    • Anti-MC4 Receptor (extracellular) Antibody
    Accession (Uniprot) Number P32245
    Gene ID 4160
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 13/15 amino acid residues identical.
    RRID AB_2039980.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-MC4 Receptor (extracellular) Antibody
      Western blot analysis of rat brain lysates:
      1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).
      2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    • Anti-MC4 Receptor (extracellular) Antibody
      Western blot analysis of mouse brain lysates:
      1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).
      2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-MC4 Receptor (extracellular) Antibody
      Expression of MC4R in mouse brain
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:100). MC4R (green) is expressed in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    • Anti-MC4 Receptor (extracellular) Antibody
      Expression of MC4R in rat pituitary cell line
      Cell surface detection of MC4R in live intact GH3 cells with Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:50). A. MC4R staining was observed, followed by Alexa-555-conjugated goat anti-rabbit secondary antibody (red). Hoechst 33342 (blue) is used to visualize the nuclei. B. Live view of the same field as A.
    References
    1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
    2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. 284, E468.
    3. Wikberg, J.E. et al. (2000) Pharmacol. Res. 42, 393.
    4. Huszar, D. et al (1997) Cell 88, 131.
    5. Adan, R.A. et al. (2006) Br. J. Pharmacol. 149, 815.
    Scientific background

    Melanocortin receptor 4 (MC4R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3

    One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1

    All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC4R activation is α-MSH = ACTH > β-MSH >> γ-MSH. AGRP and agouti/ASP are both endogenous antagonists of MC4R.1,2,3

    MC4R is widely expressed in the brain including the cortex, thalamus, hypothalamus, and brain stem. The best understood physiological role of MC4R is in the regulation of feeding behavior and the control of body weight. Indeed, mice with a targeted disruption of the MC4R gene display an obese phenotype characterized by increased adiposity, hyperphagia, and hyperleptinaemia.4 Similarly, MC4R mutations in humans have been associated with severe childhood obesity including characteristics very similar to the MC4R knockout mouse phenotype.5 All these make MC4R an attractive drug target for the treatment of obesity.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-MC4 Receptor (extracellular) Antibody
    Expression of MC4R in rat hippocampus. Immunohistochemical staining of rat brain sections using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024). MC4R staining (brown) is detected in CA1 region. Adapted from Massey, A.T. et al. (2016) Front. Neurol. 7, 65. with permission of Frontiers.
    Last update: 05/03/2020

    Anti-MC4 Receptor (extracellular) Antibody (#AMR-024) is a highly specific antibody directed against an epitope of the human melanocortin receptor 4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It recognizes an extracellular epitope and can potentially recognize the receptor in living cells. It has been designed to recognize MC4R from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Anti-MC4 Receptor (extracellular) Antibody
      Expression of MC4R in mouse hippocampal neurons.
      A. Immunocytochemical staining of mouse hippocampal neurons. Extracellular staining of cells using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024) (green). B. Western blot analysis of MC4R-transfected HEK 293 cells treated with shRNA targeting MC4R. shRNA treatment decreased the receptor level by 83%. MC4R protein levels were detected with Anti-MC4 Receptor (extracellular) Antibody.
      Adapted from Shen, Y. et al. (2013) with permission of the Society for Neuroscience.
    Immunohistochemistry citations
    1. Rat brain sections.
      Massey, A.T. et al. (2016) Front. Neurol. 7, 65.
    Immunocytochemistry citations
    1. Mouse hippocampal neurons.
      Shen, Y. et al. (2013) J. Neurosci. 33, 464.
    More product citations
    1. Peng, C.Y. et al. (2012) J. Neurosci. 32, 17211.
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