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Anti-MERTK (extracellular) Antibody

Receptor tyrosine kinase MerTK, C-Mer Proto-Oncogene Tyrosine Kinase, Mer, Proto-Oncogene C-Mer
Cat #: ATR-033
Alternative Name Receptor tyrosine kinase MerTK, C-Mer Proto-Oncogene Tyrosine Kinase, Mer, Proto-Oncogene C-Mer
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KPPIKRQDGELVGYR, corresponding to amino acid residues 402 - 416 of mouse MERTK (Accession Q60805). Extracellular, N-terminus.
Accession (Uniprot) Number Q60805
Gene ID 17289
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 13/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1, 2. Anti-MERTK (extracellular) Antibody (#ATR-033), (1:400).
    3, 4. Anti-MERTK (extracellular) Antibody, preincubated with MERTK (extracellular) Blocking Peptide (#BLP-TR033).
  • Western blot analysis human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), mouse BV-2 microglia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6):
    Western blot analysis human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), mouse BV-2 microglia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6):
    1-3. Anti-MERTK (extracellular) Antibody (#ATR-033), (1:400).
    4-6. Anti-MERTK (extracellular) Antibody, preincubated with MERTK (extracellular) Blocking Peptide (#BLP-TR033).
Immunohistochemistry
  • Expression of MERTK in mouse hippocampus
    Expression of MERTK in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MERTK (extracellular) Antibody (#ATR-033), (1:300), followed by goat anti-rabbit-AlexaFluor-488 (green). A. MERTK staining (green) in the mouse hippocampal dentate gyrus region is detected in blood vessel profiles (arrows) in the hilus (H) and outer molecular layer (OML). B. Pre-incubation of the antibody with MERTK (extracellular) Blocking Peptide (#BLP-TR033), suppresses staining. Cell nuclei are stained with DAPI (blue). G = Granule layer.
  • Expression of MERTK in rat cerebellum
    Expression of MERTK in rat cerebellum
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MERTK (extracellular) Antibody (#ATR-033), (1:300), followed by goat anti rabbit conjugated with Alexa 488 (green). A. MERTK immunoreactivity appears in in blood vessel profiles (vertical arrows) in the molecular (M), purkinje (P) and granule (G) layers and in basket profiles (horizontal arrows) around Purkinje soma. B. Pre-incubation of the antibody with MERTK (extracellular) Blocking Peptide (#BLP-TR033), suppresses staining. Cell nuclei are stained with DAPI (blue). G= Granule layer, P = Purkinje layer, M = Molecular layer.
Indirect flow cytometry
  • Cell surface detection of MERTK in live intact mouse BV-2 microglia cell line:
    Cell surface detection of MERTK in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-MERTK (extracellular) Antibody (#ATR-033), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Graham, D.K. et al. (2014) Nat. Rev. Cancer 14, 769.
  2. Kasikara, C. et al. (2017) Mol. Cancer Res. 15, 753.
  3. Huelse, J.M. et al. (2020) Pharmacol. Ther. 213, 107577.
  4. Cummings, C.T. et al. (2013) Clin. Cancer Res. 19, 5275.
Scientific background

Myeloid-epithelial-reproductive tyrosine kinase, MERTK, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and MERTK) family, expressed in numerous cancers1.

The extracellular N-terminal domain contains two immunoglobulin-like and two fibronectin 3 domains, followed by a hydrophobic single pass transmembrane domain, and a cytoplasmic C-terminal tail containing a tyrosine kinase domain1,2.

Auto-phosphorylation induced by ligand binding influences a range of downstream signaling pathways and cellular functions2. The physiological roles of MERTK include regulation of tissue homeostasis and repair, innate immune control, and platelet aggregation. However, aberrant expression of this protein promotes neoplasia, cell cycle progression, proliferation and tumor growth, resistance to apoptosis, and promotion of tumor metastases.

MERTK is involved in the activation of several canonic oncogenic signaling pathways in various types of cancers including: leukemia, non-small cell lung cancer, glioblastoma, melanoma, prostate cancer, breast cancer, colon cancer and gastric cancer1,3,4.

Due to its pivotal role in cancer development and metastasis, multiple therapeutic approaches are currently in development to inhibit MERTK, including ligand "traps" and small-molecule tyrosine kinase inhibitors4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 21/10/2020

Anti-MERTK (extracellular) Antibody (#ATR-033) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize MERTK from human, rat, and mouse samples.

For research purposes only, not for human use
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