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Anti-mGluR2 (extracellular) Antibody

Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B

Cat #: AGC-011
Alternative Name Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide SLSRGADGSRHIC, corresponding to amino acids 109-121 of rat mGluR2 (Accession P31421). Extracellular, N-terminus.
Accession (Uniprot) Number P31421
Gene ID 24415
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human, dog, monkey - identical.
RRID AB_2039987.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat cerebellum (lanes 1 and 3) and cortex (lanes 2 and 4) membranes:
    Western blot analysis of rat cerebellum (lanes 1 and 3) and cortex (lanes 2 and 4) membranes:
    1,2. Anti-mGluR2 (extracellular) Antibody (#AGC-011), (1:400).
    3,4. Anti-mGluR2 (extracellular) Antibody, preincubated with mGluR2 (extracellular) Blocking Peptide (#BLP-GC011).
  • Western blot analysis of mouse brain membranes:
    Western blot analysis of mouse brain membranes:
    1. Anti-mGluR2 (extracellular) Antibody (#AGC-011), (1:200).
    2. Anti-mGluR2 (extracellular) Antibody, preincubated with mGluR2 (extracellular) Blocking Peptide (#BLP-GC011).
Immunohistochemistry
  • Expression of mGluR2 in rat brain
    Expression of mGluR2 in rat brain
    Immunohistochemical staining of perfusion-fixed brain frozen sections using Anti-mGluR2 (extracellular) Antibody (#AGC-011). A. mGluR2 (green) is visualized in the corpus callosum (CC) and hippocampal stratum oriens (OR). B. Glial fibrillary acidic protein (GFAP) (red), a marker of astrocytes. C. Merge of the two images demonstrates expression of mGluR2 in astrocytes. DAPI is used as the nuclear counterstain (blue).
Live cell imaging / Immunocytochemistry
  • Expression of mGluR2 in rat PC12 cells
    Expression of mGluR2 in rat PC12 cells
    Cell surface detection of mGluR2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-mGluR2 (extracellular) Antibody (#AGC-011), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
References
  1. Flor, P.J. et al. (1995) Eur. J. Neurosci. 7, 622.
  2. Conn, P.J. and Pin, J.P. (1997) Annu. Rev. Pharmacol. Toxicol. 37, 205.
  3. Swanson, C.J. et al. (2005) Nat. Rev. Drug Discov. 4, 131.
Scientific background

L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/Go and hence inhibit cAMP formation following receptor activation.1,2

mGluR2 is widely distributed throughout the brain with high expression in several limbic areas including the cortex, hippocampus and amygdala. mGluR2 is localized primarily presynaptically, although postsynaptic localization has also been described.

In line with its presynaptic localization, mGluR2 is thought to function as an autoreceptor in a negative feedback mechanism that suppress further release of glutamate from the cell on which it is expressed.

The involvement of mGluR2 in neuronal excitability and synaptic transmission suggests that modulation of this receptor is a promising strategy for the treatment of neurological and neuropsychiatric disorders such as anxiety, schizophrenia, and pain.3,4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat mGluR2. The epitope is specific for mGluR2 and will not cross-react with the closely related mGluR3 channel. Anti-mGluR2 (extracellular) Antibody (#AGC-011) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize mGluR2 from human, rat and mouse samples.

For research purposes only, not for human use
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