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Guinea pig Anti-mGluR5 (extracellular) Antibody

Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Cat #: AGP-090
Alternative Name Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424). Extracellular, N-terminus.
Accession (Uniprot) Number P31424
Gene ID 24418
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/14 amino residues identical.
RRID AB_2756619.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1,2. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGP-090), (1:400).
    3,4. Guinea pig Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
     
    Following a broad screen of secondary antibodies, the following were used for this application:
    #106-035-006 (Jackson ImmunoResearch) 
    #A7289 (Sigma)
References
  1. Dore, A.S. et al. (2014) Nature. 511, 557.
  2. Chen, C. et al. (2012) Neurosci. Lett. 524, 65.
  3. Cleva, R.M. and Olive, M.F. (2011) Molecules 16, 2097.
Scientific background

Metabotropic glutamate G protein-coupled receptors are divided into three groups on the basis of their sequence similarity, pharmacology and signal transduction mechanisms. mGluR5 is a class I mGlu receptor and is widely expressed throughout the cortex, hippocampus, striatum, caudate nucleus and nucleus accumbens, areas involved in emotion, motivation and cognition. mGluR5 is primarily located post-synaptically and is coupled to the Gq/11 pathway.

mGluR5 is comprised of an extracellular N-terminus, an intracellular C-terminus and seven transmembrane α helices (TM1–7). Native ligands can bind to the receptor’s extracellular domain. This extracellular binding is consistent with the narrow allosteric pocket the receptor possesses due to the tight configuration of its helical bundle1. mGluR5 is physically coupled to NMDA receptors by various scaffolding proteins including PSD-95Shank, and Homer, as well as via a direct interaction. In addition, mGluR5 is biochemically coupled to NMDA receptor function via PKC. As a result of these molecular and biochemical interactions, activation of mGluR5 results in enhanced functionality of the NMDA receptor.

mGluR5 is also involved in dendrogenesis and spineogenesis. mGluR5−/− knockout mice exhibit elevated spine densities irrespective of spine type or location along their dendritic trees in comparison to mGluR5+/− animals2.

Recent studies have shown that mGluR5 is a critical mediator and a potential therapeutic target for the treatment of numerous disorders of the central nervous system, including depression, anxiety, drug addiction, chronic pain, Fragile X syndrome, Parkinson’s disease, and gastroesophageal reflux disease3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat mGluR5. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGP-090), raised in guinea pigs, can be used in western blot analysis. It has been designed to recognize mGluR5 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-mGluR5 (extracellular) Antibody (#AGC-007) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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