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Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody

Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Cat #: AGC-007-AR
Alternative Name Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424). Extracellular, N-terminus.
Accession (Uniprot) Number P31424
Gene ID 24418
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/14 amino residues identical.
RRID AB_2340945.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGC-007), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*, lci*
Immunohistochemistry
  • Expression of mGluR5 in rat hippocampus
    Expression of mGluR5 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:50). A. mGluR5 (red) is detected in pyramidal layer neurons (arrow). B. DAPI is used to visualize cell nuclei (blue). C. Merge of A and B.
  • Multiplex staining of NK1 Receptor and mGluR5 in rat DRG
    Multiplex staining of NK1 Receptor and mGluR5 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion using Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody (#ATR-001-AG), (green), (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (red), (1:60). A. NK1 receptor staining. B. mGluR5 staining appears in both cells (horizontal arrow) and fibers (vertical arrow). C. Merge of A and B demonstrates the co-localization of NK1 and mGluR5 receptors. Nuclei staining using DAPI as the counterstain (blue).
  • Multiplex staining of TRPV1 and mGluR5 in rat DRG
    Multiplex staining of TRPV1 and mGluR5 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:60). A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C. Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).
References
  1. Kew, J.N.C. and Kemp, J.A. (2005) Psychopharmacology (Berl). 179, 4.
  2. Ferraguti, F. and Shigemoto, R. (2006) Cell Tissue Res. 326, 428.
  3. Dhami, G.K. and Ferguson, S.S. (2006) Pharmacol. Ther. 111, 260.
Scientific background

L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.

mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.

mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.

The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson’s and Huntington’s disease.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-mGluR5 (extracellular)-ATTO Fluor-594 AntibodyMultiplex staining of TRPV2 and mGluR5 in rat DRGImmunohistochemistry of rat dorsal root ganglion using Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033) (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR) (1:60). A. TRPV2 staining (green). B. mGluR5 staining (red). C. Merge of A and B demonstrates co-localization of TRPV2 and mGluR5 in DRG cells. Nuclei are stained using DAPI as the counterstain (blue).

Last update: 16/08/2020

Anti-mGluR5 (extracellular) Antibody (#AGC-007) is a highly specific antibody directed against an extracelluar epitope of rat mGluR5. The antibody can be used in western blot analysis, immunhistochemical and live cell imaging applications. It has been designed to recognize mGluR5 from rat, mouse and human samples.

Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry and is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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