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Anti-mGluR5 (extracellular)-FITC Antibody

Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Cat #: AGC-007-F
Alternative Name Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424). Extracellular, N-terminus.
Accession (Uniprot) Number P31424
Gene ID 24418
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/14 amino residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGC-007), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells:
    Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells:
    ___ Cells.  
    ___ Cells + rabbit IgG isotype control-FITC.  
    ___ Cells + Anti-mGluR5 (extracellular)-FITC Antibody (#AGC-007-F), (5 µg).
References
  1. Kew, J.N.C. and Kemp, J.A. (2005) Psychopharmacology (Berl). 179, 4.
  2. Ferraguti, F. and Shigemoto, R. (2006) Cell Tissue Res326, 428.
  3. Dhami, G.K. and Ferguson, S.S.G. (2006) Pharmacol. Ther. 111, 260.
Scientific background

L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.

mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.

mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.

The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, inflammation, chronic pain, Parkinson’s and Huntington’s disease.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-mGluR5 (extracellular) Antibody (#AGC-007) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize mGluR5 from rat, mouse, and human samples.

Anti-mGluR5 (extracellular)-FITC Antibody (#AGC-007-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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