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Anti-MRGPRE Antibody

Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167

Cat #: AMR-062
Alternative Name Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE (Accession Q7TN40). 3rd intracellular loop.
Accession (Uniprot) Number Q7TN40
Gene ID 404660
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 10/13 amino acid residues identical.
RRID AB_2756665.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6) brain membranes:
    Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6) brain membranes:
    1-3. Anti-MRGPRE Antibody (#AMR-062), (1:400).
    4-6. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
  • Western blot analysis of human U-87 MG glioblastoma:
    Western blot analysis of human U-87 MG glioblastoma:
    1. Anti-MRGPRE Antibody (#AMR-062), (1:400).
    2. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Immunohistochemistry
  • Expression of MRGE in rat hippocampus
    Expression of MRGE in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MRGPRE Antibody (#AMR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. MRGE staining (green) in hippocampal dentate gyrus is detected in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Bader, M. et al. (2014) Pharmacol. Rev. 6, 1080.
  2. Solinski, H.J. et al. (2014) Pharmacol. Rev. 66, 570.
Scientific background

The Mas-related G protein-coupled receptors (MRGPRs), formerly called Mas-related genes (MRGs) includes nine members: MrgprA, B, C, D, E, F, G, H, and the primate-specific MRGPRX1,2.

MRGPRE consist of only one receptor per species and is conserved in rodents and primates2. MRGPRE structure contains a Cys-Cys bridge from transmembrane domain (TM) 3, an “ionic-lock” known to inhibit activation and activity in some class A GPCRs1,2.

Human and mouse MRGPRE is highly expressed in neurons of the brain and mast cells. Transcripts are also found in medium and large diameter neurons of human dorsal root ganglia (DRG) sections and in other areas of the central nervous system, including cerebral cortex, hippocampus, spinal cord, and cerebellum. MRGPRE was also found in human, mouse, and rat placenta tissue and in enteric neurons1,2.

Mouse lacking the MRGPRE protein showed decreased nociceptive behavior and significant deficits in the induction of mechanical allodynia after chronic constriction injury2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-MRGPRE Antibody (#AMR-062) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRE from rat, human, and mouse samples.

For research purposes only, not for human use
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