Overview
- Peptide (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE (Accession Q7TN40). 3rd intracellular loop.
Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6) brain membranes:1-3. Anti-MRGPRE Antibody (#AMR-062), (1:400).
4-6. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Western blot analysis of human U-87 MG glioblastoma:1. Anti-MRGPRE Antibody (#AMR-062), (1:400).
2. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Expression of MRGE in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MRGPRE Antibody (#AMR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. MRGE staining (green) in hippocampal dentate gyrus is detected in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).
- Bader, M. et al. (2014) Pharmacol. Rev. 6, 1080.
- Solinski, H.J. et al. (2014) Pharmacol. Rev. 66, 570.
The Mas-related G protein-coupled receptors (MRGPRs), formerly called Mas-related genes (MRGs) includes nine members: MrgprA, B, C, D, E, F, G, H, and the primate-specific MRGPRX1,2.
MRGPRE consist of only one receptor per species and is conserved in rodents and primates2. MRGPRE structure contains a Cys-Cys bridge from transmembrane domain (TM) 3, an “ionic-lock” known to inhibit activation and activity in some class A GPCRs1,2.
Human and mouse MRGPRE is highly expressed in neurons of the brain and mast cells. Transcripts are also found in medium and large diameter neurons of human dorsal root ganglia (DRG) sections and in other areas of the central nervous system, including cerebral cortex, hippocampus, spinal cord, and cerebellum. MRGPRE was also found in human, mouse, and rat placenta tissue and in enteric neurons1,2.
Mouse lacking the MRGPRE protein showed decreased nociceptive behavior and significant deficits in the induction of mechanical allodynia after chronic constriction injury2.
Anti-MRGPRE Antibody (#AMR-062) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRE from rat, human, and mouse samples.
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