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Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody

Sodium/hydrogen exchanger 3, SLC9A3

Cat #: ANX-033
Alternative Name Sodium/hydrogen exchanger 3, SLC9A3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide (C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3 (Accession P26433). Intracellular, C-terminus.
Accession (Uniprot) Number P26433
Gene ID 24784
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 13/14 amino acid residues identical; human – 12/14 amino acid residues identical.
RRID AB_2756738.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat small intestine lysate:
    Western blot analysis of rat small intestine lysate:
    1. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033), (1:200).
    2. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody, preincubated with Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033).
  • Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:
    Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:
    1. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033), (1:200).
    2. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody, preincubated with Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033).
References
  1. Counillon, L. and Pouyssegur, J. (2000) J. Biol. Chem. 275, 1.
  2. Donowitz, M. et al. (2013) Mol. Aspect. Med. 34, 236.
  3. Sarker, R. et al. (2011) Am. J. Physiol. 300, C771.
  4. He, P. et al. (2015) J. Clin. Invest. 125, 3519.
Scientific background

Maintaining physiological intracellular pH values is vital for most cells. The Na+/H+ exchanger family (NHE, also known as SLC9) is widely expressed and has a key role in protecting cells against acidification. There are currently six NHE transporters identified in mammalian cells1.
 
The complete structure of members of the NHE family has not been completely solved. However, all members are organized in a similar manner with 11 or 12 membrane spanning domains, carrying out the Na+ and H+ exchange and a varying length intracellular C-terminal domain being involved in regulation of the exchange activity. The transport of Na+ and H+ in NHEs occurs in the center of the N-terminus via a two inverted funnel area that is made up of two transmembrane regions.
 
NHE3 is expressed in sodium absorptive cells of the mammalian small intestine, colon, gall bladder, renal proximal tubule, and thick and thin limbs of the loop of Henle and can be mostly found in the plasma membrane2.
 
NHE3 interacts with a multi-PDZ domain-containing scaffold protein called NHERF-1. NHERF-1 binds to the NHE3 C-terminus and has a role in NHE3 regulation in various cells. NHERF-1 functions as a cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP3. In addition, silencing of NHERF-1 in a model of diabetic mice was found to prevent NHE3 trafficking and insulin-dependent activation4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NHE-3. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033) can be used in western blot analysis. It has been designed to recognize NHE-3 from human, mouse and rat samples.

For research purposes only, not for human use
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