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- Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
- Expression of NaV1.1 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-SCN1A (NaV1.1)-ATTO-594 Antibody (#ASC-001-AR). A. Staining of NaV1.1 (red) is mainly in the molecular layer (Mol). B. DAPI is used as the counterstain (blue). C. Merge images of A and B.
- 1. Wu, L. et al. (2002) NeuroReport 13, 2547.
- 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
- 3. Fjell, J. et al. (2000) NeuroReport 11, 199.
- 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
- 5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
- 6. Isom, L.L. (2001) Neuroscientist 7, 42.
- 7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
- 8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768.
- 9. Rhodes, T.H. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 101, 11147.
Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.
Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7.
NaV1.1, also referred to as SCN1A, is a tetrodotoxin-sensitive channel and is broadly expressed in neurons7.
Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI)8,9.
Species reactivity key:
Anti-SCN1A (NaV1.1) Antibody (#ASC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.1 from rat, human, and mouse samples.
Anti-SCN1A (NaV1.1)-ATTO-594 Antibody (#ASC-001-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-SCN1A (NaV1.1)-ATTO-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.
- Guinea pig Anti-SCN1A (NaV1.1) Antibody (#AGP-043) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #ASC-001.
- Blockers/Antagonists: peptides/peptide toxinsBlockers/Antagonists: small moleculesActivators/Agonists: peptides/peptide toxinsActivators/Agonists: small molecules