Guinea pig Anti-SCN2A (NaV1.2) Antibody

BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
    Cat #: AGP-026
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
    Accession (Uniprot) Number P04775
    Gene ID 24766
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical.
    NaV1.3 - 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
    1,2. Guinea pig Anti-SCN2A (NaV1.2) Antibody (#AGP-026), (1:1000).
    3,4. Guinea pig Anti-SCN2A (NaV1.2) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Immuno-colocalization of Melatonin receptor 2 and SCN2A in rat paraventricular nucleus
    Immunohistochemical staining of perfusion-fixed frozen rat paraventricular nucleus sections using Anti-Melatonin Receptor Type 2 Antibody (#AMR-032), (1:600) and Guinea pig Anti-SCN2A (NaV1.2) Antibody (#AGP-026), (1:2000). A. Melatonin Receptor Type 2 staining (red) (arrows). B. The same section labeled for SCN2A (green). C. Merge of A and B demonstrates partial co-localization of Melatonin Receptor Type 2 and SCN2A in the paraventricular nucleus (PVN). For orientation, note location with respect to the third ventricle (3rd V). Cell nuclei were stained with DAPI (blue).
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. et al. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
    8. Catterall, W.A. et al. (2008) J. Neurosci28, 11768.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

    Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

    NaV1.2, also known as SCN2A, is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the NaV1.2 channel have been identified in different types of epilepsy.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NaV1.2 channel. Guinea pig Anti-SCN2A (NaV1.2) Antibody (#AGP-026) raised in guinea pigs can be used in western blot and immuhistochemistry applications. It has been designed to recognize NaV1.2 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-SCN2A (NaV1.2) Antibody (#ASC-002) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use
    Citations
    Related Products
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        1. Anti-SCN2A (NaV1.2)-ATTO-594 Antibody (#ASC-002-AR). A fluorescent labeled primary antibody. It can be used in colocalization studies in conjunction with any of our antibodies raised in rabbit.