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Guinea pig Anti-NaV1.5 (SCN5A) Antibody

Sodium channel protein type 5 subunit alpha, SKM2
Cat #: AGP-008
Alternative Name Sodium channel protein type 5 subunit alpha, SKM2
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: h, m, r
Immunogen
  • Peptide DRLPKSDSEDGPRALNQLSC, corresponding to amino acid residues 493-511 of rat NaV1.5 (Accession P15389). Intracellular loop between domains I and II.
Accession (Uniprot) Number P15389
Gene ID 25665
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 17/19 amino acid residues identical.
RRID AB_2340960.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.95 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of human-NaV1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4):
    Western blot analysis of human-NaV1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4):
    1,2. Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#AGP-008), (1:200).
    3,4. Guinea pig Anti-NaV1.5 (SCN5A) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).
Immunohistochemistry
  • Expression of NaV1.5 in rat heart
    Expression of NaV1.5 in rat heart
    Immunohistochemical staining of rat heart frozen sections using Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#AGP-008). NaV1.5 staining (red) appears in cardiomyocytes and in intercalated discs (arrows). Nuclei are stained using DAPI (blue).
  • Human heart sections (1:200) (Cheng, J. et al. (2017) Sci. Rep. 7, 42953.).
References
  1. Wu, L. et al. (2002) Neuroreport. 13, 2547.
  2. Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.
Scientific background

Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability1.

Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.

The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.52.

The putative structure of NaV1.5 consists of four homologous domain (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome1,2. Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Guinea pig Anti-Nav1.5 (SCN5A) Antibody
Multiplex staining of NaV1.5 and Vinculin in human heart.Immunohistochemical staining of human ventricle heart sections using Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#AGP-008) and mouse anti-vinculin antibody. NaV1.5 staining (green) is detected in intercalated disks and co-localizes with vinculin.Adapted from Cheng, J. et al. (2017) Sci. Rep. 7, 42953. with permission of SPRINGER NATURE.
Last update: 16/08/2020

Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#AGP-008), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Human heart sections (1:200).
    Cheng, J. et al. (2017) Sci. Rep. 7, 42953.
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